未知病变类型脑血管中β-amyloid、α-actin、collagen Ⅳ的含量

Immunohistochemical Study on Contents of β-amyloid, α-actin and Collagen Ⅳ in Cerebral Small Vessels with Unknown Type of Pathological Lesion

目的研究未知病变类型脑血管病变的结构特征。方法通过刚果红染色、免疫组织化学染色、计算机图像分析技术对未知病变类型脑血管病变的β-amyloid、α-actin、collagenⅣ的含量进行研究。结果未知病变类型脑血管壁α-actin、collagenⅣ呈少量阳性染色,与正常脑血管存在显著差异(P<0.05);β-amyloid染色呈阴性,与正常脑血管无差异(P>0.05)。病变血管壁中上述三种蛋白的表达特点与脑血管淀粉样变(cerebralamyloidangiopathy,CAA)及小动脉硬化玻璃样变不同。结论未知病变类型脑血管病变具有不同于CAA的病变特征。

Objective To investigate the structural characteristics of the cerebral small vessels with an unknown type of pathological lesion （UTPL）. Methods Contents of β-amyloid, α-actin and collagen Ⅳ in cerebral small vessels with UTPL were studied by Congo red staining, immunohistochemical staining and computer image analysis. Results The low expression levels of α-actin and collagen Ⅳ （P〈0.05）were observed in tunica media of the vessels with UTPL, and no positive expression of β-amyloid（P〉0.05） was observed in these vessel walls. The expressions of proteins mentioned above in UTPL were different from those of cerebral amyloid angiopathy（CAA） and hyaline arteriolosclerosis. Conclusion UTPL was different from CAA or hyaline arteriolosclerosis in pathologic feature.