摘要
研究了大球盖菇(Stropharia rugoso-annulata)菌丝原生质体制备和再生条件,结果表明:在30℃、pH5.5、0.6mol/L甘露醇、1.5%溶壁酶条件下对培养3d的菌丝体酶解2.5h,原生质体产量达到1.36×107个/mL。原生质体适宜再生培养基为马铃薯200g,蔗糖20g,蛋白胨2g,酵母粉2g,KH2PO41.5g,K2HPO41.5g,MgSO4.7H2O 1.5g,维生素B10.1g,维生素B60.1g,0.6mol/L甘露醇。采用上述培养基,双层平板法进行再生试验,再生率达到0.96%。为大球盖菇原生质体技术深入研究和应用奠定了基础。
The conditions of protoplast preparation and regeneration from the mycelia of Stropharia rugoso - annul.am were studied. Results showed that the 3 - day - old myceha,when subjected to 1.5% Lywallzyme solution in 0.6mol/L annitol （pH value 5.5） at 30℃ for 2.5 hours with gentle shaking, produced an efficient yield of protoplasts（ 1.36 × 10^7/mL）. The medium with the following composition： potato 200g, sucrose 20g, peptone 2g, yeast extracts 2g, KH2 PO4 1.5g, K2HPO4 1.5g , MgSO4·7H2O 1.5g, vitamin B1 0. 1g, vitamin B6 0.1g, 0.6mol/L mannitol was suitable for protoplast regeneration. The highest protoplast regeneration rate （0.96%）was obtained when incubated on two layer plates of the
出处
《生物技术》
CAS
CSCD
2006年第6期57-60,共4页
Biotechnology
基金
山东省教育厅资助项目(J03K36)
关键词
大球盖菇
原生质体
形成
再生
Stropharia rugoso- annulata
protoplast
preparation
regeneration
