摘要
观察了不同浓度的水杨酸(salicylicacid,SA)对豇豆枯萎病病菌的孢子萌发、菌丝生长、感病品种挑战接种后幼苗发病率、β-1,3-葡聚糖酶和几丁质酶酶活性变化的影响。结果表明:大于2.0mmol/L的SA溶液都能显著地抑制豇豆枯萎病病菌孢子的萌发;在1.0~10.0mmol/L范围内,SA浓度越大对菌丝的抑制作用越强;在不影响豇豆感病品种幼苗正常生长的情况下,以5.0mmol/L的SA最为有效,幼苗发病率仅为23.2%;感病品种经SA处理后引起了β-1,3-葡聚糖酶和几丁质酶活性的升高,挑战接种后的酶活性变化比未经SA处理的上升早且快。
Salicylic acid(SA) at different concentration was tested on conidial germination and mycelial growth of yardlongbean fusarium wilt fungus, disease rate, chitinase and β-1, 3-glucosanase activity of seedling after challenge inoculation in the experiment. The results indicated:that SA at the concentration of above 2.0 mmol/L had inhibition to the conidial germination. At 1.0- 10.0 mmol/L SA had inhibition to the mycelial growth. Without any harm to the seedling, treatment with SA at 0.5 mmol/L was most effective and reduced the infection rate by 63.2 %. SA enhanced the activity of the two enzymes in the susceptible cultivar,compared to the plants without SA treatment. In the plants with prior SA treatment β-1,3-glucosanase and chitinase were activated significantly earlier when the host plants were further challenged by the pathogen.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2006年第6期610-613,共4页
Journal of Huazhong Agricultural University
基金
广东省农业科技攻关项目(200218120034155)资助
关键词
豇豆枯萎菌
水杨酸
孢子萌发
菌丝生长
Β-1
3-葡聚糖酶
几丁质酶
yardlong bean fusarium wilt fungus
salicylic acid
conidial germination
mycelial growth
β-1,3-glucosanase
chitinase