机械损伤和茉莉酸甲酯对烟株蛋白酶抑制剂的诱导作用研究

Research on the mechanical wounding and methyl jasmonate induced augment of proteinase inhibitors in tobacco

为了进一步研究蛋白酶抑制剂对昆虫生长发育的影响,选取机械损伤和茉莉酸甲酯作为信号路径的激发子,研究了它们在不同条件下诱导烟株局部和系统性的胰蛋白酶抑制剂和胰凝乳蛋白酶抑制剂的表达。结果显示:机械损伤或茉莉酸甲酯处理后,烟株处理叶和系统叶中胰蛋白酶抑制剂和胰凝乳蛋白酶抑制剂含量都有不同程度升高;施用2.4mmol/L茉莉酸甲酯时两者的增加量最为显著;机械损伤能明显抑制2.4mmol/L茉莉酸甲酯的诱导作用,对1.2mmol/L茉莉酸甲酯具有协同作用。

Proteinase inhibitor is one of the most important compounds in plants, which can effectively resist the growth and propagation of insects. Mechanical wounding and methyl jasmonate （MeJA） were chosen as elicitors of the response pathway to investigate the augment of trypsin proteinase inhibitor （TI） and chymotrypsin proteinase inhibitor （CI） in tobacco. Results showed that mechanical wounding or MeJA can induce local and systemic increases of both TI and CI in different levels. Maximum levels of TI and CI in tobacco leaves would be reached when tobacco plants were treated with 2.4 mmol/L MeJA. Moreover, mechanical wounding can affect the production of TI and CI in a positive way when the plants were treated with 1.2 mmol/L MeJA while in a negative way with 2.4 mmol/L MeJA.