大黄素对人肺腺癌细胞Anip973增殖周期及凋亡基因的影响

Effects of Emodin on Proliferation Cycle and Apoptotic Gene of Human Lung Adenocarcinoma Cell Line Anip 973

目的探讨大黄素对人肺腺癌Anip973细胞生长的抑制作用及其对细胞增殖周期和凋亡基因的影响。方法采用甲基噻唑(MTT)比色法和生长曲线测定不同浓度大黄素在不同作用时间内对在体外培养的Anip973细胞增殖的影响,同时应用流式细胞术检测细胞增殖周期及凋亡率的变化,用免疫印迹法检测Caspase-3蛋白表达。结果大黄素可在G0/G1期阻滞人肺腺癌Anip973细胞的增殖,使S期细胞比率降低,并激活Caspase-3蛋白;在一定范围内,大黄素的浓度越高、作用时间越长,对肿瘤细胞生长的抑制作用越强,凋亡率也越高。结论大黄素能抑制人肺腺癌Anip973细胞生长,其抗肿瘤机制是通过激活Caspase-3,诱导细胞凋亡而阻止细胞周期。

Objective To study the suppressive role of emodin on the growth and its effect on the proliferation cycle and apoptotic gene of human lung adenocarcinoma cell line Anip 973. Methods The survival rate and the inhibitory rate of Anip 973 cell in vitro were detected by MTT colorimetric assay and cell growth curve assay at different time points under different concentration of emodin; the cell proliferation cycle and the apoptotic rate were examined with flow cytometry analysis, and Caspase-3 protein expression was measured by immunoblotting assay. Results Emodin inhibited the proliferation of Anip 973 cell at G0/G1 phase, decreased the cell ratio at S phase and activated the Caspase-3 protein. It suppressed the growth of tumor cells and raised the apoptotic rate in a concentration and time depending manner in a certain extent. Conclusion Emodin could suppress the proliferation of Anip 973 cell, and its mechanism of anticancer effect may be through activating Caspase-3, to induce apoptosis and block cell cycle.