自制肺炎衣原体单克隆抗体检测其特异性抗原对动脉粥样硬化诊断的临床意义

Clinical Value of Detecting Chlamydophila Pneumoniae Specific Antigen

目的探讨采用自制肺炎衣原体单克隆抗体(Cpn-McAb)检测人外周血单核细胞(PBMC)和冠状动脉瘤标本中肺炎衣原体特异性抗原(Cpn-Ag)的临床意义。方法采用自制Cpn-McAb的直接免疫荧光(DI)法检测颈动脉粥样硬化和健康体检者PBMC中Cpn-Ag及冠状动脉瘤和正常冠状动脉标本中Cpn-Ag的表达。结果颈动脉粥样硬化患者和健康体检者PBMC中Cpn-Ag检测结果间差异有显著性意义(P<0.01);粥样硬化冠状动脉瘤标本和正常冠状动脉标本外膜下和/或斑块内Cpn-Ag检测结果间差异有显著性意义(P<0.01)。冠状动脉瘤标本中有大量Cpn-Ag沉积,分别位于外膜下及粥样硬化斑块内,而正常血管壁中仅有少量沉积(P<0.05)。结论PBMC中的Cpn-Ag的检测可为诊治与该病原体相关的疾病提供依据;Cpn可能是动脉粥样硬化形成的原因之一。

Objective To explore the clinical significance of detecting Chlamydophila pneumoniae specific antigen （Cpn -Ag） in peripheral blood mononuclear cells （PBMC） and expression of Cpn - Ag in coronary artery atheroma by selfmade monoclonal antibody to Chlamydia pneumoniae （ Cpn - McAb）. Methods The human PBMC was separated and Cpn - Ag was measured by direct immunofluorescence （DI） test, at the same time, the expression of Cpn -Ag was detected by D1 using selfmade Cpn - McAb for coronary artery atheroma specimens and normal coronary artery specimens. Results There was a significant difference in Cpn - Ag level of the PBMC between the patients with increased interma - media thickness （IMT） of the carotid artery and those in control group （ P 〈0. 01 ）. There was a significant difference in positive rate of Cpn - Ag between coronary artery atheroma specimens and normal artery specimens （ P 〈 0. 01 ）. A lot of apple - green dots were located in atherosclerotic plaques and in adventitia of coronary artery atheroma specimens, but only a little dots were found in adventitia of normal artery specimens （ P 〈 0. 05）. Conclusion There were high positive rate of Cpn - Ag in PBMC of patient with increased IMT of the carotid artery and high expression rate of Cpn - Ag in adventitia of coronary artery atheroma. The Cpn may be one of the eausational factors for the formation of lesions within the coronary artery atherosclerosis.