VEGF-C MMP9及TIMP-1在宫颈癌组织中的表达及临床意义

Expression of Vascular Endothelial Growth Factor C,MMP9 and TIMP1 and the Clinical Significance in Cervical Carcinoma

目的:探讨血管内皮生长因子(VEGF-C)、基质金属蛋白酶MMP9及其抑制物TIMP-1在宫颈癌组织中的表达,及与宫颈癌临床病理特征的关系。方法:采用免疫组化链霉菌抗生物素蛋白-过氧化物酶连接染色法(SP法),检测62例宫颈癌Ⅰ、Ⅱ期患者癌组织中VEGF-C、MMP9及其抑制物TIMP-1的表达,并进行统计学分析。结果:VEGF-C、MMP9及其抑制物TIMP-1在宫颈鳞癌Ⅰ、Ⅱ期均为高表达。VEGF-C在宫颈癌Ⅰ、Ⅱ期和有淋巴转移者的表达率分别为73.8%、85%、100%。MMP9为66.7%、75%、93.3%。TIMP-1为64.3%、60%、66.7%,VEGF-C、MMP9及TIMP-1在浸润间质≥1/2的表达高于<1/2(P<0.05)者;其表达与浸润深度及淋巴结转移相关。结论:VEGF-C与宫颈癌的侵袭和转移密切相关;MMP9及其抑制物TIMP-1与肿瘤的浸润及侵袭转移有关,VEGF-C、MMP9及TIMP-1在宫颈癌转移中发挥了重要作用。

Objective： To investigate the expression of vascular endothelial growth factor C （VEGFC）, matrix metalloproteinase 9 （MMP9） and tissue inhibitor of metalloproteinase 1 （TIMP1） in cervical cancer tissue and the relationship between these levels and the elinicopathologic characteristics of cervical carcinoma. Methods： The expression of VEGFC, MMP9 and TIMP1 in 62 patients with cervical carcinoma of stage- Ⅰ and Ⅱ were determined using immunohistoehemistry （SP method） and statistical analysis was then conducted. Results： There were high expression rates for VEGFC （77.4%）, MMP9（69.4%）, and TIMP1（62.9%） in the cases of squamous cancer （stage- Ⅰ and Ⅱ ）. The expression rate of VEGFC in the stage- Ⅰ and Ⅱ cases and in the cases with lymph node metastasis was 73.8%, 85% and 100.0%, respectively, while that of MMP9 was 66.7%, 75% and 93.3% and that of TIMP1 was 64.3%, 60% and 66.7%, respectively. The expression of VEGFC, MMP9 and TIMP1 was higher as the depth of stromal infiltration by the cervical carcinoma was equal to or more than 1/2, compared to that of tumors with an infiltration depth of less than 1/2 （P〈0.05）. There was a correlation between the expression rates and the depth of infiltration as well as lymphatic metastasis. Conclusions： Our research indicates that there is a close correlation between expression of VEGFC, MMP9 and TIMP1 and tumor cell invasion and metastasis. VEGFC, MMP9 and TIMP1 may play important roles in the development and aggressiveness of human cervical carcinoma.