甾体激素对绒毛外滋养细胞表达HLA-G蛋白的影响

Effect of Steroid Hormone on the Expression of HLA-G Protein in Human Extravillous Trophoblasts

目的:探讨甾体激素(E_2、P_4)对绒毛外滋养细胞表达HLA-G的影响。方法:将分离培养的绒毛外滋养细胞(EVT)分成E_2组、孕酮(P_4)组、E_2+P_4组和空白对照组,分别向各组EVT培养液中加入不同浓度的17β-雌二醇、孕酮及17β-雌二醇+孕酮,空白对照组则不加任何激素,48h后,流式细胞仪检测各组HLA-G蛋白的表达。结果:①单独使用E_2或P_4均能上调EVT表达HLA- G蛋白,上调作用与其浓度关系密切,与对照组相比,差异有显著性(P＜0．01);②联合应用E_2+P_4亦上调EVT表达HLA-G蛋白,1000 nmol/L的E_2+P_4组HLA-G蛋白的表达,明显高于低浓度组(1nmol/L、10nmol/L、100nmol/L),差异有显著性(P＜0．01);③低浓度的E_2+P_4组(1nmol/L)HLA- G蛋白的表达高于同浓度的P_4组(P＜0．05),而低于同浓度的E_2组(P＜0．001);1000nmol/L,E_2+P_4组HLA-G表达显著高于单纯的E_2、P_4组(P＜0．01)。结论:①低浓度的E_2、P_4对HLA-G调节呈拮抗作用,高浓度E_2和P_4则以协同作用调节HLA-G的表达;②HLA-G可能参与了卵巢甾体激素调节胚胎植入进程。

Objective： To explore the regulation ofhorm one on the expression of HLA-G. Methods： After extravillous cytotrophoblasts （EVT） were isolateel and cultured, beta-estradiol （E2）, progesterone（P4） and E2＋P4 were added to E2, P4 and E2＋P4 group respectively with the final concentration varying from 1 nmol/L, 10 nmol/L, 100 nmol/L to 1 000 nmol/L. After 48 h, EVT were removed to investigate the expression of membrance-binding HLA-G protein by flow cytometry. Results： 1） Either E2 or P4 can up-regulate the expression of HLA-G protein with physiological dose in culture. And the difference between E2 group or P4 group was significant compared with control group after 48 h （P〈0.01）. 2） There was a significant increase of HLA-G levels with E2 and P4 in culture at any concentration, and the levels continue increasing. However, significant increases in HLA-G protein levels were only observed in EVT treated with the higher concentrations of E2 and P4 （1 000 nmol/L）. While with the lower concentration （1 nmol/L）, HLA-G protein levels were higher than those in control group and P4 group, but lower than those in E2 group. There was no significance in EVT treated with 10 nmol/L and 100 nmol/L of E2 and P4 among E2, P4 group and E2＋P4 group. Conclusion： 1） Steroid hormone （E2 and P4） regulated HLA-G level in antagonistic action way; but in a joint action way with higher concentration.2） HLA-G may play some role in the process of steroid hormone of ovary during the embryonic implantation process.