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恩替卡韦对慢性乙型肝炎患者树突状细胞功能的体外影响 被引量:13

In vitro effect of entecavir on the function of dendritic cells derived from chronic hepatitis B patients
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摘要 目的:研究恩替卡韦(ETV)对慢性乙型肝炎(CHB)患者外周血树突状细胞(dendritic cell,DC)功能的影响.方法:体外常规分离CHB患者及健康人外周血单个核细胞,诱导扩增后常规培养.第4天将其与一定浓度的恩替卡韦共培养,第8天收获DC进行细胞表型、同种异体混合淋巴细胞反应等相关检测.结果:细胞培养8 d时DC形态分化健康对照组优于CHB ETV处理组,CHB ETV处理组优于CHB组;CHB组CD1a(35.73±3.12 vs 62.31±5.22,P<0.01),CD80(28.19±1.64 vs 45.38±3.10,P<0.01),CD83(22.24±2.14 vs 40.63±7.21,P<0.01)及HLA-DR(36.74±0.98 vs 56.05±3.89,P<0.01)表达明显低于健康对照组,而ETV处理组与CHB组相比CD83 (27.41±9.23 vs 22.24±2.14,P<0.05),CD80(32.67±7.82 vs 28.19±1.64,P<0.05)及HLA-DR(40.84±5.57 vs 36.74±0.98,P<0.01)显著高表达;淋巴细胞增殖能力测定ETV处理组DC刺激同种异体T淋巴细胞增殖能力较CHB组增强(1.53±0.09 vs 1.45±0.12,P<0.05).结论:恩替卡韦作为治疗CHB的新一代核苷类药物,除了直接抑制乙肝病毒DNA合成外,也能够增强CHB患者外周血DC的功能,通过调节机体的免疫系统发挥间接抗病毒作用. AIM: To investigate the effect of entecavir (ETV) on the function of dendritic cells (DC) derived from patients with chronic hepatitis B (CHB) in vitro. METHODS: Peripheral blood mononuclear cells were isolated from CHB patients and healthy controls. After proliferation under induction, the cells were cultured by routine method. Then ETV was added to co-incubate with the cells on the 4^th day. On day 8, DC were collected for the detection of phenotypes and mixed lymphocytic reactions. RESULTS: After 8 days of cultivation, the differentiation of DC in the healthy controls was better than that in ETV-treated group, while ETV-treated group had a better differentiation than CHB group. In comparison with those in the healthy controls, the levels of CD1a (35.73±3.12 vs 62.31 ± 5.22), CD80 (28.19 ± 1.64 vs 45.38 ± 3.10), CD83 (22.24 ± 2.14 vs 40.63 ± 7.21) and HLA-DR (36.74 ± 0.98 vs 56.05 ± 3.89) expression were significantly decreased in CHB group (all P 〈 0.01). However, the levels of CD83 (27.41 ± 9.23 vs 22.24 ± 2.14, P 〈 0.05), CD80 (32.67 ± 7.82 vs 28.19 ± 1.64, P 〈 0.05) and HLA-DR (40.84 ± 5.57 vs 36.74 ± 0.98, P 〈 0.01) expression were markedly increased in ETV-treated group as compared with those in CHB group. The capacity to stimulate proliferation of the allogeneic lymphocytes in ETV-treated group was higher than that in CHB group (1.53 ± 0.09 vs 1.45 ± 0.12, P 〈 0.05). CONCLUSION: Entecavir can enhance the biological activity of DC derived from CHB patients and play an indirect antivirus function by regulating the host's immune system.
出处 《世界华人消化杂志》 CAS 北大核心 2007年第11期1292-1296,共5页 World Chinese Journal of Digestology
基金 河南省卫生厅医学创新人才工程项目 No.2004-47~~
关键词 慢性乙型肝炎 树突状细胞 恩替卡韦 细胞表型 混合淋巴细胞反应 Chronic hepatitis B Dendritic cell Entecavir Cell phenotype Mixed lymphocytic reaction
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