摘要
采用流式细胞仪检测1、3、5、7、14、21、28、35、42、49日龄AA肉鸡血液中的CD3、CD4、CD8阳性T细胞比例。研究结果表明:1~5日龄T细胞逐渐进入血液参与细胞免疫,7、21日龄注射疫苗起免疫应答作用,28日龄后基本形成稳固的细胞免疫水平。
In order to verify the dynamic rules of T lymphocyte and its subsets in AA broiler, lymphocytes were separated from chicken blood and the concentration of CD4^+ and CD8^+ subsets of T cells and CD3^+ T cells were checked with flow cytometer in different old days of AA broiler. The results indicated that the T cells entered into blood and joined in cell-mediated immunity at 1 to 5 days old. The first vaccination came into protecting at 7 days old, Then the second vaccination brought immunoreactions at 21 days old. The immunity became steady after 28 days old.
出处
《中国家禽》
北大核心
2007年第11期12-13,17,共3页
China Poultry
基金
国家自然科学基金资助项目(30160067)