缺氧对海马神经元培养的影响及巴曲酶的保护作用—HSP70的表达及细胞形态学的研究

Batroxobin against anoxia damage of rat hippocampal neurons in culture —Morphological changes and HSP70 expression

本文采用新生大鼠海马神经元培养技术，以细胞形态学及ＨＳＰ７０免疫阳性细胞表达为指标，首次观察了巴曲酶对缺氧海马神经元损伤的直接保护作用。结果发现：在缺氧前０．５ｈ给予巴曲酶（０．５ＢＵ／ｍｌ），海马神经细胞存活率以及ＨＳＰ７０免疫阳性细胞率均明显高于缺氧对照组（Ｐ＜０．０１），而在缺氧前２４ｈ给予巴曲酶后，二者与缺氧对照组均无明显差别。表明：巴曲酶对缺氧海马神经细胞损伤有直接的保护作用，而且其作用与给药的时机有关。

Neurotransmitter Research Laboratory,Chinese PLA General Hospital,Beijing 100853. We have previously found that batroxobin has neuroprotective effect in ischemic reperfusion rats.The purpose of this study was to obtain further information on the mechanism of the batroxobin induced neuroprotection.Therefore,the influence of batroxobin on cultured hippocampal neurons of natal rat after two hours anoxia followed by 48 hours reoxygenation was investigated by using morphological changes and heat shock protein 70KD (HSP70) expression as indicators.We found that batroxobin given 24 hours before anoxia had no effect on neurons in culture.However,batroxobin given 30 minutes before anoxia followed by 48 hours reoxygenation could greatly increase in survival rate and number of HSP70 positive neurons of hippocampus as compared with anoxic controls.The results suggest that batroxobin may have a direct neuroprotective effect against anoxic damage of hippocampal neurons.