转铁蛋白正选择位点的检测及分析

Detection and Analysis of Positive Selection Sites at the Transferrins

利用密码子替换模型和最大似然法模型,检测位点间的正选择作用并分析了转铁蛋白分子适应性进化。结果显示:保守的转铁蛋白受到正选择作用。其中,正选择位点大多集中于转铁蛋白C臂的二级结构无规则区域,即非α螺旋也非β折叠区域,而少数位于N臂。有证据认为这些正选择位点可能与转铁蛋白N臂和C臂结合铁离子功能有关。

The codon -substitution and maximum likelihood models were used to detect positive selection effect among sites and analyze the molecular adaptive evolution of transferrins. The result shows that conservative transferfins suffered the positive selection effect. Most of the positive selection sites are focus on the random coils of C - lobe of transfelTins, which secondary structure not the alpha helix or the beta sheet. Few positive selection sites location on the N - lobe. There are some experimental evidences to consider that these positive selection sites may influence the difference of bind iron between the N -lobe and C -lobe of transferrins.