高胆血红素血症新生儿细胞因子表达水平的探讨

Discussion of the expressed level of TNF-α,TNF-β,GM-CSF and sIL-2R in neonate with hyperbilirubinemia

目的:探讨某些细胞因子在病理性高胆血红素血症新生儿中临床意义。方法:将病理性高胆血红素血症新生儿分为3组,无菌采集病理性高胆血红素血症新生儿股动脉血清,均采用双抗体夹心ABC-ELISA法,测定TNF-α、TNF-β、GM-CSF、sIL-2R的表达水平。结果:①高胆一组与高胆二组细胞因子比较,仅GM-CSF有显著性统计学意义(P<0.01)。②高胆一组与高胆三组细胞因子比较,仅TNF-α有显著统计学意义(P<0.01)。③高胆二组与高胆三组细胞因子比较,TNF-α、TNF-β、GM-CSF有显著统计学意义(P<0.01)。④高胆三个组与正常组比较,TB IL、DB IL、TNF-α、TNF-β、GM-CSF均有显著统计学意义(P<0.01),仅sIL-2R无统计学意义(P>0.05)。结论:TNF-α、TNF-β、GM-CSF可做为病理性高胆血红素血症新生儿诊疗指标,为围产期病理性高胆血红素血症新生儿早期干预及其保健提供基础免疫学理论依据。

Objective： To discuss the clinic value of some cytokines in neonate with hyperbilirubinemia. Methods： All neonates with hyperbilirubinemia were divided into three groups. The expressed level of TNF - α, TNF - β, GM - CSF, slL -2R were measured in aseptic collection serum from arteria cruralis in neonate using double antibody binding ABC - ELISA reagent from RD company in USA. Results： ①There was significant difference in GM - CSF between group one and group two （P 〈0. 01）. ②There was significant difference in TNF - α between group one and group three （P 〈0. 01 ）. ③There was significant difference in TNF - α, TNF - β and GM - CSF between group two and group three （P 〈0. 01 ）. ④There was significant difference in TBIL, DBIL, TNF - α, TNF - β and GM - CSF among the three groups with hyperbilirubinemia （P 〈0. 01 ）, only slL -2R had no significant difference among the three groups with hyperbilirubinemia （P 〉 0. 05） . Conclusion ： TNF - α, TNF - β and GM - CSF can be the new marker for diagnose and treatment for neonates with patho-hyperbilirubinemia to provide the basic immunology evidence for early intervention and health maintenance for neonates with patho -hyperbilirubinemia during perinatal period.