液态生产胞外漆酶大型真菌高产菌株筛选

Screening of effective macro-fungi strains with high extracellular laccase-productivity

依照真菌漆酶催化特定作用底物进行氧化还原反应结果的特性,设计了一个采用愈创木酚和α-萘酚作为酶反应显色判别依据和产酶菌株筛选的限制性驯化剂,同时结合选用木质素作为产酶诱导强化因子的筛选分析模版,对保藏和外购的常见大型担子菌和食用真菌进行产酶性能分析。结果发现,这些菌株均不能有效地生产漆酶或酶活性很低,以该模版作为初筛手段从杨树林等野生菌菇生长地中分离得到15株产酶野生微生物,经反复筛选得到3株酶活性较高的大型真菌株A21、X18和Y11,其胞外漆酶活力分别为255.9、248.7和277.3U.ml-1,具有可观的产业化生产开发应用潜力。

Based on the redox reaction of special substrates catalyzed by fungal laccase, an effective screening method for isolating laccase-producing macro-fungi strains was designed. Guaiacol and a-naphthol were used for color-developing in determining this enzymatic reaction and as the factors for restrictive selection, and lignin was used as an inducer to enhance the enzymatic productivity. The extracellular laccase-productivity of general macro-fungi and edible mushroom strains preserved in our laboratories or purchased from other institutions and supermarkets was investigated, but none of the strains could effectively produce laccase, or the produced laccase had very low activity. Taking our method as the means for primary isolation, 15 potent laccase-producing macro-fungi strains were successfully isolated from wild woody districts like poplar forest land. Three high-efficient macro-fungi strains, A21, X18 and Y11, were further screened, with the extracellular laccase activities being 255.9, 248.7 and 277.3 U U.ml^-1, respectively, which could be used as the strains with considerable potential of industrialization.