硅油对兔眼角膜内皮结构的影响

Effect of silicone oil on corneal endothelium of rabbit eyes

目的：制备前房内硅油注入和硅油注入取出的动物模型,以探讨实验动物眼中硅油对角膜内皮的影响,同时观察、分析硅油取出后角膜的病理变化。方法：将40只健康成年白兔随机分成3组：前房硅油注入组（SIG）、前房硅油注入取出组（SIOG）、空白对照组（CG）。将SIG及和SIOG制成动物模型后进行裂隙灯、角膜厚度、角膜内皮细胞密度、角膜组织学及透射电镜检查,分析硅油在前房内允许停留的最长时间及前房硅油注入取出后角膜发生的病理变化。结果：角膜内皮细胞密度检查结果中,SIG和SIOG 8-16周时角膜内皮细胞密度比相应时限CG下降明显（P〈0.01）;组织学及透射电镜下可见12周角膜内皮细胞核固缩,损伤区出现多层角膜内皮细胞。SIOG组在裂隙灯下角膜呈水肿状,SIOG组角膜厚度与SIG组比较明显增加（P〈0.01）,组织学及透射电镜结果显示角膜基质的纤维束排列疏松、紊乱,角膜基质厚度明显增加。CG组角膜内皮细胞无显著改变。结论：兔眼前房内硅油停留8周可引起角膜内皮细胞发生不可逆的损伤,时间越长,损伤越严重。

Objective To investigate the effect of silicone oil on the corneal endothelium in experimental animal models, while the silicone oil in the anterior chamber of the experimental animals will be taken out so as to explore their corneal changes. Methods Forty healthy white rabbits were randomly divided into three groups ： silicone oil in the anterior chamber group （SIG）, silicone oil in and out of the anterior chamber group （SIOG） and control group （CG）. The cornea of rabbit was investigated at different times by slit-lamp, corneal thickness instrument, endothelial cell count instrument, histology and electromicroscopy. The time of silicone oil in the anterior chamber without keratpathy and the pathological changes of cornea with taking out of the silicone oil were observed. Results Thedensity of the corneal endothelium in SIOG and SIG decreased more obviously by endothelial cell count instrument from 8 weeks to 16 weeks compared with control group （P〈0. 01）. The results of histology and electron microscopy showed that the core of cells shrank, many layers of corneal endothelium had been found. Cornea of SIOG was edematous by the slit-lamp. Compared with SIG, the corneal thickness in SIDG increased significantly （P〈0. 01） ;the fibers of corneal stroma in SIOG were loose and disorder. The corneal stroma thickness in SIOG increased lightly （P〈0. 01）. The cornea in CG didn＇ t change obviously. Conclusion The damage of corneal endothelium of rabbit eyes will not recover if the silicone oil is in the anterior chamber beyond 8 weeks, more time come with more damage.