新疆欧洲鳞毛蕨中总生物碱的提取及抑菌活性研究

Study on Extraction and Anti-microbial Activities of Total Alkaloids from Dryopteris filix-mas(L.) Schott

目的研究欧洲鳞毛蕨中总生物碱体外抑菌活性,为合理开发利用欧洲鳞毛蕨提供理论依据。方法超声辅助提取欧洲鳞毛蕨中的总生物碱,采用滤纸片法测定总碱抑菌活性,琼脂平板稀释法进行MIC的测定,欧洲鳞毛蕨总生物碱具有较强的抑菌作用。结果欧洲鳞毛蕨总生物碱对供试菌种的最低抑菌浓度分别为:大肠埃希杆菌0.8 mg/mL,金黄色葡萄球菌0.4 mg/mL,枯草杆菌1.6 mg/mL,奇异变形杆菌1.6 mg/mL,酵母3.2 mg/mL。结论欧洲鳞毛蕨总生物碱具有较强的抑菌作用,对细菌、酵母都具有一定的抑菌作用,开发前景乐观。

Objective The anti-microbial activities of the alkaloid extracted from Dryopteris filix-mas（L.） Schott are studied in this paper.Methods Total alkaloids were extracted from Dryopteris filix-mas（L.） by ultrasound-assisted extraction.The methods of filter paper piece was taken to determine anti-microbial activities of total alkaloids from Dryopteris filix-mas（L.） and agar plate dilution method to detect MIC of total alkaloids from Dryopteris filix-mas（L.）.Results The MIC of total alkaloids were 0.8 mg/mL,0.4 mg/mL,1.6 mg/mL,1.6 mg/mL,3.2 mg/mL,respectively.Conclusions The total alkaloids have great strong anti-microbial activities.It has exploitation prospect.