化疗药物对高表达SNCG的乳腺癌细胞株T47D的抑制作用的实验研究

Experimental research on the inhibition of chemotherapeutic drugs to the breast cancer cell line T47D with high expression of SNCG

目的:探讨各种化疗药物对高表达γ突触核蛋白(SNCG)的乳腺癌T47D细胞株的抑制作用。方法:常规培养不表达及高表达SNCG的乳腺癌MCF-7及T47D细胞株,采用MTT法观察顺铂(DDP)、阿霉素(ADM)、5-氟尿嘧啶(5-FU)、长春新碱(VCR)、紫杉醇(TAX)、依立替康(CPT-11)对细胞的抑制作用,并用流式细胞术分析药物作用后的细胞周期变化及细胞凋亡。结果:作用60 h后,ADM、VCR、TAX及CPT-11对T47D细胞的抑制作用显著低于MCF-7细胞(P<0.01),而DDP、5-FU对T47D细胞的抑制作用与MCF-7细胞相比差异无显著性(P>0.05),且显著高于其他4种抗肿瘤药物(P<0.01)。药物作用T47D细胞60 h后,DDP、5-FU组的细胞增殖指数(PI)及Caspase-3表达均较对照组及其他4组差异有极显著性(P<0.01)。结论:DDP及5-FU对高表达SNCG的T47D细胞的抑制作用显著高于ADM、VCR、TAX及CPT-11,此可为临床对乳腺癌患者个体化治疗方案的选择提供体外实验依据。

Objective：To explore the inhibition of different chemot herapeutic drugs to the breast cancer T47D cell line which highly expressesγ-synuclein（SNCG）.Metbods：T47D with SNCG expression and MCF-7 without SNCG expression cell lines were routinely cultured.MTT method was employed to evaluate the inhibiting roles of 6 chemotherapeutic drugs（DDP,ADM,5-FU, VCR,TAX and CPT-11）to the two cell lines.Moreover,Flow cytometry was enrolled to analyze the cell cycles and the apoptosis of T47D cell lines.Results：The inhibiting roles of ADM,VCR,TAX and CPT-11 to T47D were much lower than that of MCF-7 cell line after 60 h treatment（P〈0.01）,while the effects of 5-FU and DDP to T47D cell line were not only the same as to MCF-7,but also much higher than the other 4 drugs（P〈0.01）.Moreover,there were significant differences of PI and caspase-3 expressions of T47D cell line between DDP,5-FU groups and control or other 4 groups,respectively（P〈0.01）.Conclusion：The inhibiting effects of DDP,5-FU to T47D cell line with high expression of SNCG are much higher than those of ADM,VCR,TAX and CPT-11,which can provide experimental evidence in vitro for the chemotherapy strategy to breast cancer patients.