抑制血吸虫排卵对实验感染兔循环表膜抗原检测的影响

EFFECT OF INHIBITION OF WORM OVULATION IN SCHISTOSOMA JAPONICUM-INFECTED RABBITSON THE DETECTION OF CIRCULATING MEMBRANE ANTIGEN

目的 :了解循环表膜抗原阳性反应与血吸虫雌虫排卵的关系。方法 :用新西兰兔 15只 ,各经肤接种日本血吸虫尾蚴 2 50条 ,于感染后不同时间口服丙烯基硫脲 ,对比感染前、后逐周血中用斑点 - EL ISA法测出的循环表膜抗原。结果 :第一组兔感染 19d后开始口服丙烯基硫脲 2 95-590 mg/ d,每周连续喂药 3d直至感染后第 8wk,循环表膜抗原始终为阴性。第二组兔于第 4 6d起服同剂量 ,感染后 6wk时斑点 - EL ISA呈阳性反应持续 2 wk。第三组对照兔于感染后 6wk开始亦出现持续 2 wk的斑点 - EL ISA阳性反应。结论 :循环表膜抗原阳性反应与雌虫排卵密切相关。

AIM:To understand the effect of inhibiting worm ovulation on the detection of circulat- ing membrane antigen in Schistosoma japonicum infected rabbits. METHODS:Fifteen New Zealand rabbits were each infected with2 5 0 S.japonicum cercariae per cuttem and a chemi- cal,allyl thiourea,which inhibit egg formation was administrated per os at days1 9and46 after infection. Sera were collected from rabbits before and weekly after infection and sub- jected to dot- ELISA for circulating antigen detection.RESULTS:Group1 rabbits were ad- ministered allyl thiourea2 95 - 5 90 mg/d1 9d post infection(p.i.) on3consecutive days per wk until 8wk p.i.,no circulating membrane antigen could be detected in the blood stream throuhgout the experiment.Group2 rabbits received the same dose of allyl thiourea as that in group1 starting from d46 p.i..Dot- ELISA positive reaction was obtained6 wk p.i.and lasted for 2 wk. In Group 3untreated infected rabbits,dot- EL ISA positive reaction was also detected at6 wk p.i.and lasted for2 wk.CONCLUSION:The positive circulating membrane antigen reaction is closely related to egg laying by the female worms.