摘要
为了探讨热激蛋白在B型烟粉虱Bemisiatabaci B-biotype抵抗高温中的作用,以含有B型烟粉虱热激蛋白基因hsp70片段的质粒为模板,用TaqMan-MGB探针和特异性引物构建了B型烟粉虱hsp70实时荧光定量RT-PCR检测体系,并检测了37℃-45℃高温胁迫及气温变化时B型烟粉虱成虫体内hsp70表达的情况。结果表明：缓和的高温对B型烟粉虱成虫hsp70表达具有诱导作用,在37℃-41℃范围内,hsp70的表达量随着温度的升高从8.78×10^5±6.41×10^4拷贝数上升到1.99×10^7±1.45×10^5拷贝数,在41℃时达到最高峰;而当温度升高到43℃和45℃,hsp70表达量迅速降低。B型烟粉虱成虫hsp70的表达与昼夜气温变化有关,当上午气温从34℃上升到41℃时,hsp70的表达量从1.16×10^5±1.48×10^4拷贝数上升到6.29×10^6±1.80×10^5拷贝数;当傍晚气温下降到33℃时,hsp70表达量也下降到2.32×10^5±7.69×10^3拷贝数。因此推测,hsp70在B型烟粉虱抵抗高温胁迫方面可能具有重要作用。
The plasmid with hsp70 cDNA of Bemisia tabaci B-biotype was used as a template. Based on the GenBank sequence data, one TaqMan-MGB probe and the corresponding primers were designed to construct a standard for real-time fluorescence quantitative RT-PCR to detect the expression of hspTO in B. tabaci B- biotype, with which the changes in expression level of hsp70 from adults of B. tabaci B-biotype under different high temperature stress from 37℃to 45 ℃ were detected, The results showed that mild high temperature could induce hspTO expression in B. tabaci B-biotype adults. Within the range of 37 ℃ to 41 ℃, the hsp70 expression level of B. tabaci B-biotype adults increased from 8.78 × 10^5 ± 6.41 × 10^4 copies to 1.99× 10^7 ± 1.45 × 10^5copies; However, when the temperature increased to 43℃ and 45℃, the hsp70 expression level decreased very quickly. The hsp70 expression level of B. tabaci B-biotype adults changed in response to the diurnal temperature change. When air temperature increased from 34℃ to 41℃ in the morning, the hsp70 expression level increased dramatically from 1.16 × 10^5 ± 1.48 × 10^4 copies to 6.29 × 10^6 ± 1.80 × 10^5 copies; however, the hsp70 expression level decreased to 2.32 × 10^5 ±7.69 × 10^3 copies when air temperature decreased to 33℃ at nightfall. It was so inferred that hsp70 in B. tabaci B-biotype may play an important role in high temperature tolerance.
出处
《昆虫学报》
CAS
CSCD
北大核心
2007年第11期1087-1091,共5页
Acta Entomologica Sinica
基金
国家重点基础研究发展规划"973"项目(2002CB111400)
