中药降脂颗粒对非酒精性脂肪肝大鼠肝脏瘦素受体mRNA及P-JAK2/P-STAT3的影响

Effect of Jiangzhi granules on expression of leptin receptor mRNA,P-JAK2 and P-STAT3 in rats with non-alcoholic fatty liver disease

目的:观察降脂颗粒对非酒精性脂肪肝大鼠的治疗作用及其对血清瘦素、肝组织瘦素受体mRNA、P-JAK2/P-STAT3蛋白含量表达的影响.方法:将60只SD♂大鼠,随机分为空白组(正常饮食)和造模组(高脂饮食).待造模成功后将造模组大鼠随机分为模型对照组、降脂颗粒低、中、高剂量组和东宝甘泰组.治疗4 wk后行肝组织生化和病理学检测,并同时应用ELISA试剂盒检测血清瘦素,RT-PCR法检测肝组织瘦素受体mRNA表达,应用Western blot检测肝组织P-JAK2和P-STAT3蛋白的表达.结果:低、中、高剂量组降脂颗粒能明显降低非酒精性脂肪肝大鼠模型肝脂(TG和TC)水平,改善脂肪变性,降低肝脏炎症反应,并能明显降低血清瘦素高水平状态(193.02±23.8ng/ L,163.97±31.38ng/L,147.83±17.59ng/L vs 317.22±39.26ng/L,P<0.01),改善瘦素抵抗,同时增加瘦素受体mRNA的表达(1.87±0.06,2.20±0.04,2.78±0.04 vs 1.50±0.05,P<0.01),增加肝组织P-JAK2和P-STAT3蛋白的含量(119.88±2.98,123.45±0.68,124.34±3.42 vs 113.15±1.27,P<0.01;94.15±0.78,100.18±3.33,101.94±2.20 vs 89.06±0.69,P<0.01).结论:降脂颗粒对非酒精性脂肪肝大鼠肝脏脂质和炎症有较好的治疗作用,其可能机制是改善瘦素抵抗,增加肝脏瘦素受体mRNA表达及P-JAK2,P-STAT3蛋白含量.

AIM： To study the effect of Jiangzhi granules on non-alcoholic fatty liver disease in rats, and on the expression of leptin receptor mRNA, P-JAK2 and P-STAT3 in the liver. METHODS： Sixty-eight male Sprague-Dawley rats were randomly divided into two groups： blank group （n = 8）, fed a normal diet; Model group （n = 60）, fed high fat diet, Fifty-eight rats in Model group were randomly divided into five groups： control group （n = 10）. Jiangzhi granules low-dose group （n = 12）, Jiangzhi granules moderate dose group （n = 12）, Jiangzhi granules high-dose group （n = 12） and Dongbao Gantai （DB） group （n = 12）. We examined the effect of traditional Chinese medicine and DB on liver/ body weight ratio, expression of leptin receptor mRNA （RT-PCR）, P-JAK2 （Western blotting）, P-STAT3 （Western blotting）, triglyceride （TG） and total cholesterol （TC） in liver tissue. RESULTS： In the low-, moderate- and high- dose groups, Jiangzhi granules significantly reduced liver lipid （TG, TC） levels, improved steatosis, reduced hepatitis, and significantly reduced the high level of serum leptin （193.02 ± 23.8 ng/L, 163.97 ± 31.38 ng/L, 147.83 ± 17.59 ng/L vs 317.22 ± 39.26 ng/L, P 〈 0.01）, improved leptin resistance, increased expression of leptin receptor mRNA （1.87 ± 0.06, 2.20 ± 0.04, 2.78 ± 0.04 vs 1.50 ± 0.05, P 〈 0.01）, P-JAK2 （119.88 ± 2.98, 123.45 ± 0.68, 124.34 ± 3.42 vs 113.15 ± 1.27, P 〈 0.01） and P-STAT3 （94.15 ± 0.78, 100.18 ± 3.33, 101.94 ± 2.20 vs 89.06 ± 0.69, P 〈 0.01）. CONCLUSION： Jianghzhi granules effectively improve liver function, hepatitis and steatosis. Jiangzhi granules can increase expression of leptin receptor mRNA, p-JAK2 and p-STAT3 in liver tissue.