摘要
目的:检测胃癌组织中P27的表达及其与cyclin D_1、cyclin E表达的相关性,并探讨其意义.方法:临床病理资料齐全的胃癌蜡块标本54例,正常胃黏膜标本15例,采用SP免疫组化方法检测P27、cyclin D和cyclin E在其中的表达.结果:胃癌组织54例中有20例P27表达阳性(37.0%),正常胃组织中15例有11例P27呈阳性表达(73.3%),胃癌组织与正常胃组织相比,P27的表达有明显差异(P<0.05),胃癌组织中P27的表达与患者的性别、年龄及肿瘤大小,浸润深度,分化程度无相关性(P>0.05),而与TNM分期及有无淋巴结转移明显相关(P<0.05),P27的表达与cyclin D_1的表达呈负相关(r=-0.332),而与cyclin E的表达无明显相关性(p>0.05).结论:胃癌组织中P27表达与正常胃组织有显著差异,胃癌组织中P27蛋白表达与淋巴结转移及临床分期密切相关,与cyclin E的表达呈负相关.
AIM： To detect the expression of P27 and cyclin D1 and E expression in gastric cancer, and to provide a new way to diagnose and treat gastric cancer.
METHODS： Immunohistochemistry was used to examine the expression of P27, cyclin D1 and cyclin E proteins in gastric carcinoma （n = 54） and normal gastric mucosa （n = 15）.
RESULTS： Positive immunohistochemistry was seen in 20 of 54 gastric cancer cases and in 11 of 15 normal gastric tissues. P27 expression differed significantly between gastric cancer and normal gastric tissue （P 〈 0.05）, had no relation with sex, age, tumor size, invasive depth and differentiation, but had a significant relationship with TNM staging and lymph node metastasis （P 〈 0.05）. P27 expression had a negative correlation with cyclin D1 （r = -0.332） and no relationship with cyclin E.
CONCLUSION： The difference in P27 expression in gastric cancer and normal gastric tissue is remarkable. The expression has a significant relationship with TNM staging and lymph node metastasis. P27 expression has a negative correlation with cyclin D1 and no relationship with cyclin E expression.
出处
《世界华人消化杂志》
CAS
北大核心
2007年第36期3809-3814,共6页
World Chinese Journal of Digestology
