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成熟成骨细胞中敲除基因fgfr1小鼠的制备 被引量：6

Generation of differentiated osteoblast specific fgfr1 knockout mice
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摘要 目的制备在成熟成骨细胞中敲除基因fgfr1的特定细胞阶段性基因敲除小鼠。方法从美国NIH引进SPF级fgfr1条件性基因敲除(fgfr1flox/flox)小鼠,进行饲养、繁殖和PCR鉴定;fgfr1flox/flox小鼠与在成熟成骨细胞表达Cre重组酶的OC-Cre小鼠交配,获得在成熟成骨细胞中敲除一个基因拷贝的杂合子小鼠;杂合子小鼠之间交配,或者杂合子小鼠与fgfr1flox/flox小鼠交配,可以获得在成熟成骨细胞中敲除fgfr1基因的小鼠fgfr1△/△/OC-CreTG/+。结果成功制备在成熟成骨细胞中敲除fgfr1的细胞特异性敲除小鼠。结论成功引进fgfr1flox/flox小鼠,应用合理的基因条件性敲除繁殖策略,获得在成熟成骨细胞中敲除fgfr1的基因敲除小鼠。 Objective To obtain differentiated osteoblast-specific inactivation of fgfrl mice. Methods To obtain fgfr1^△/＋/OC-Cre^TG/＋mice, fgfr1^flow/flox mice obtained from NIH were crossed with OC-Cre mice. To obtain fgfr1^△/△/OC-Cre^TG/＋ mutant mice, fgfr1^△/＋/OC-Cre^TG/＋ further crossed with themselves or fgfr1^flow/flox mice. After fgfr1^△/△/OC-Cre^TG/＋ crossed with fgfr1^flow/flox mice, half of their offspring were mutant mice. Results Differentiated osteoblast-specific fgfr1 knockout mice were obtained. Conclusion fgfr1^△/△/OC-Cre^TG/＋ mice were obtained through proper crossing strategy, which provides a suitable platform for studying fgfrl function in bone development and fracture healing.
作者 李福兵 赵玲 鲁秀敏 余瑛 何启芬 陈锚锚 段亚琪 戚华兵 陈林
机构地区 第三军医大学大坪医院野战外科研究所全军创伤中心
出处 《第三军医大学学报》 CAS CSCD 北大核心 2008年第4期280-283,共4页 Journal of Third Military Medical University
基金 国家重点基础研究发展规划项目(“973”项目)(2005CB522604) 国家自然科学基金杰出青年基金(30425023) 国家自然科学基金重点项目(30530410)~~
关键词 FGFR1 条件性基因敲除 小鼠 骨骼发育 fibroblast growth factor receptor 1 conditional gene knockout mice bone development
分类号 R-332 [医药卫生] R322.71 [医药卫生—人体解剖和组织胚胎学]
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同被引文献49

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第三军医大学学报
2008年 第4期

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