摘要
OBJECTIVE To investigate the expression of the high mobility group boxl（HMGB1） in human cervical squamous epithelial carcinoma （CSEC） and to explore the relationship of HMGB1 expression to the differentiation degree, size, invasion and metastasis of CSEC. METHODS Immunohistochemical staining of tissue microarrays and Western blot analysis were conducted to detect the expression of HMGB1 in the following tissue samples： 30 carcinoma in situ, 90 invasive CSEC without metastasis, 30 invasive CSEC with metastasis, 30 cases of normal cervical squamous epithelia. RESULTS The positive-expression rate of HMGB1 was 58.7% （88/150） in CSEC, showing a significant difference compared to normal cervical squamous epithelia. The expression of HMGB1 was correlated with tumor size, invasion and metastasis of CSEC （respectively, P〈0.01）, but had no relationship with the degree of differentiation （P〉0.05）. CONCLUSION The over-expression of HMGB1 in CSEC might be a useful parameter as an indication of tumor invasion, metastasis, prognosis and overall biological behavior of human CSEC, as well as a noval target site for gene therapy.
目的:探讨宫颈鳞癌组织中高迁移率族蛋白HMGB1表达情况及其与肿瘤分化程度、大小、浸润和转移的关系。方法:应用组织芯片免疫组织化学方法检测了30例宫颈原位癌,90例无转移的宫颈癌,30例有转移的宫颈癌和30例正常宫颈鳞状上皮中HMGB1表达情况,并应用western blot技术定量检测其表达情况。结果:HMGB1在宫颈鳞癌组织中呈强阳性表达58.7%(88/150),正常宫颈鳞状上皮中弱表达,差异具有显著性(P<0.01),HMGB1阳性率与肿瘤的大小、浸润及转移呈正相关(P<0.01);与肿瘤分化程度无关(P>0.05)。结论:HMGB1在宫颈鳞癌组织中尤其是发生转移的宫颈鳞癌组织中呈强阳性表达,可作为宫颈鳞癌浸润、转移及预后的重要判定指标之一,并有望成为基因治疗的靶点。
