Ⅱ类主要组织相容性复合物转录激活子(MHC2TA)-168A→G多态性与冠心病的相关性研究

Study on the correlation between MHC2TA-168A→G polymorphism and coronary heart disease(CHD) in Han Chinese population

目的研究Ⅱ类主要组织相容性复合物转录激活子(MHC2TA)-168A→G多态性在中国汉族人群中的分布及与冠心病的相关性。方法采用错配聚合酶链反应-限制性片段长度多态性技术(mpPCR-RFLP),对汉族185例冠心病患者及149名正常人群MHC2TA基因-168A→G位点进行研究。结果冠心病组与对照组均检出-168AA、-168AG和-168GG基因型,MHC2TA-168A等位基因频率分别为0.724和0.802,MHC2TA-168G等位基因频率分别为0.276和0.198,冠心病组MHC2TA-168G基因型频率(0.276)明显高于对照组(0.198。OR:1.542,95%CI:1.070～2.221;P<0.05。结论MHC2TA-168A→G多态性与冠心病有关,G等位基因可能是汉族人群冠心病的遗传危险因素。

Objective To investigate the correlation between Major Histocompability Complex Class Ⅱ Transactivator （MHC2TA） - 168A→ G polymorphism and CHD in Han Chinese population. Methods Using the methods of misparing polymerase chain reaction - restriction fragment length polymorphism （mpPCR-RFLP） , followed by DNA sequencing identification, 185 CHD patients and 149 controls were analyzed for the polymorphism , genotype and allele distribution of MHC2TA - 168A→G . Results Genetypes of - 168AA, - 168AG and - 168Gg were found both in patients of CHD and those in normal control . The MHC2TA - 168A allele frequency in CHD patients and normal control was 0.724 and 0. 802 respectively , that in CHD group was much higher than that in control group（ OR：1. 542 , 95 % CI： 1. 070 - 2. 221 ; P 〈 0.05 ） . Conclusions MHC2TA - 168A→G polymorphism is associated with CHD risk of Hart Chinese population , the G allele may serve as a genetic risk factor of coronary heart disease.