同源盒基因Msx2在小鼠磨牙牙根发育早期的表达

Expression of Homobox Gene Msx2 During the Early Tooth Molar Root Development in Mice

目的:观察在小鼠的牙根发育早期同源盒基因Msx2的表达,以探讨其在此过程中所起的作用。方法:出生后第6天和第8天的小鼠,取含有下颌第一磨牙的下颌骨,进行固定和脱钙,制备5μm的石蜡切片,原位杂交方法检测Msx2在小鼠牙根发育早期的表达。结果:第6天Msx2阳性信号出现在Hertwig’s上皮根鞘细胞、上皮根鞘周围的牙乳头细胞和早期的成牙本质细胞中;第8天在Hertwig’s上皮根鞘细胞、上皮根鞘周围的牙乳头细胞和成牙本质细胞中Msx2有阳性信号,而在成牙骨质细胞中无表达。结论:Msx2参与调控牙根早期发育的生理过程。

Objective： To observe the expression of Homeobox gene Msx2 and study its functions in early stages of tooth root development in postnatal mice. Methods： Mice of 6 and 8 postnatal days were sacrificed. The mandibles including the first molar were dissected and fixed. After decalcification, 5p^m thickness serial paraffin sections were prepared for in situ hybridization. Results： At postnatal 6 days, Msx2 positive signals were observed in the Hertwig＇s epithelial root sheath, dental follicle and early odontoblasts around root sheath. At postnatal 8 days, but Msx2 positive signals appeared in the Hertwig~ epithelial root sheath, dental follicle and odontoblasts around root sheath, not in cementoblasts. Conclusion： The results demonstrate that Msx2 might be involved in the early tooth root development and play an important role.