摘要
利用AFLP技术对24份红肉桃和5份白肉桃对照品种进行了DNA多态性分析,从64对E+2/M+3引物组合中筛选出6对引物用于扩增基因组DNA,共获得清晰可辨的174个标记,其中多态性标记为108个,多态性检出率为62.1%。UPGMA聚类分析结果显示,在遗传相似系数0.916处,红肉品种与白肉品种分别聚成了两大类(第6类和第7类),说明两类群间存在较明显的差异;红肉桃14、粘核红肉桃、黑桃1、黑桃2、红肉桃2与红肉桃4几个红肉桃品种独自成类,与其他绝大部分品种遗传距离较远,具有独特的基因型,故应作为重点种质保存。
24 varieties of red-flesh peach and 5 varieties of white-flesh peach were analyzed by AFLP markers, Six pairs of primer were selected from 64 pairs of primer （E＋2/M＋3） to amplify the genomic DNA. Total 174 AFLP bands were obtained, 108（62.1%） of them were polymorphic markers. UPGMA clustering result showed that when genetic similarity coeffi- cient was 0.916, red-flesh peach and white-flesh peach were clustered into two groups （group six and group seven）, showed that there was great genetic difference between the two groups. Hongroutao 14, Nianhehongroutao, Heitao 1, Heitao 2, Hongroutao 2 and Hongroutao 4 were clustered its own group separately, and distinguished from each other. Besides, some primitive state and unique genotypes were preserved in those varieties. Therefore, they should be preserved as an important germplasm resource.
出处
《湖北农业科学》
北大核心
2008年第2期138-141,共4页
Hubei Agricultural Sciences
基金
湖北省自然科学基金项目(2006ABA048)
