围产期母猪骨骼营养状况的研究

A Study on Skeleton Nutrition in Perinatal Sows

在广东某进出口公司猪场,随机选取体格健康、不同胎次的怀孕母猪10头,分别在产前30、20、10 d和分娩当天,以及产后5、10、20、30 d,清晨饲喂前从耳静脉采血10 mL,并迅速分离血清。测定血清中的碱性磷酸酶、羟脯氨酸、谷氨酸蛋白和钙、磷的含量,以研究该阶段母猪的骨营养状况。结果表明,整个围产期血清中碱性磷酸酶活性变化不大,且主要来源于肝脏;羟脯氨酸含量从产前30 d至产后5 d相对平稳,但产后5 d至产后20 d血清中的羟脯氨酸明显上升,产后30 d(断奶后)有所下降;血清中的谷氨酸蛋白含量在产前30 d至分娩当天,逐渐降低。只在分娩后5 d下降明显,此后血清中谷氨酸蛋白含量又逐渐升高;整个围产期血清钙含量水平基本平稳,只是在分娩当天有下降趋势,但差异不显著;而血清磷含量水平也呈平稳状态,只在产后10 d^20 d后下降明显,断奶后又恢复原来水平。

Ten healthy sows were randomly selected in an import-export company farm of Guangdong. 10 mL blood samples were obtained through each sow＇s ear vein on the 30,20,10 days before delivery,deliver day,and the 5,10,20,30^th days of postpartum before the morning feeding, and then sera were separated as soon as possible. The alkaline phosphatase （AKP）, bone ,γ-glutamicacid-containing protein （BGP）, hydroxyproline（HYP）, calcium（Ca） and phosphorus（P） in sera were determined aiming at studying the skeleton nutrition in perinatal sows. The results showed that the activity of seral AKP did not changed in the perinatal period, and it comes from liver mainly. The content of HYP was in relatively stable status from the 30^th day before delivery to the 5^th day postpartum. It rose obviously from the 5^th day to the 20^th day postpartum. It decreased again after ablactation（the 30^th day postpartum）. The seral BGP was at a low level in the prenatal period. It decreased in the 5^th day postpartum, but it rose gradually since that day. Calcium content was stable except from the delivering day when it decreased, but not significantly. Also the status of phosphorus was stable, it obviously decreased only at the 20^th day postpartum, and increased to normal level later.