高血压对大鼠脑缺血再灌注后细胞凋亡的影响

Effct of hypertension on neuronal apoptosis after cerebral ischemia reperfusion injury in rats

目的:通过观察高血压对大鼠脑缺血再灌注后神经功能和细胞凋亡的影响,探讨高血压加重缺血性脑损伤的机制。方法:将Wistar雄性大鼠随机分为肾性高血压缺血再灌注组(HIR),正常血压缺血再灌注组(IR)和假手术组(N),采用大脑中动脉线栓法造成大脑缺血再灌注模型,缺血时间为2h,再灌注24h。进行神经功能评分后取脑,采用免疫组织化学技术检测脑组织bcl-2、Bax的表达,TUNEL法检测细胞凋亡。结果:正常血压缺血再灌注组神经功能评分低于肾性高血压缺血再灌注组(P<0.05)。肾性高血压缺血再灌注组与正常血压缺血再灌注组比较,bcl-2表达明显减少(P<0.05),Bax表达明显增多(P<0.05),神经细胞凋亡明显增多(P<0.05)。结论:高血压通过增加Bax表达、抑制bcl-2表达,促进脑细胞凋亡,加重缺血再灌注后脑损伤。

Objective： To study the effects of hypertension on apoptosis of cell in rats with focal cerebral ischemia-reperfusion. Methods： Wistar male rats were divided randomly into hypertension ischemia and reperfusion group（HIR）, normaltension ischemia and reperfusion group（IR） and sham-operated group（N）. The focal cerebral ischemia model was established in Wistar rats by MCAO for 2h and reperfusion for 24h,then, neurological evaluation was carried out and the expression of bcl-2 and Bax were measured by immunohistochemistry. The apoptosis of cell was analyzed by TUNEL. Results： As compared with HIR,the neurological evaluation of IR was lower （P〈0.05）. and the expression of Bax were higher and the expression of bcl-2 were lower in IR （P〈0. 05）. Conclusion： Hypertension can inhibits the expression of bcl-2,and upregulate the expression of Bax in rats with focal cerebral ischemia-reperfusion.