摘要
应用超高效液相色谱-串联质谱仪(UPLC-MS-MS)研究了草莓中杀虫双残留的测定方法。样品用甲醇-水(1:1,V/V)提取,无需经过任何净化过程;以等梯度流动相、经ACQUITY UPLC HSS T3超高效液相色谱柱分离;以电喷雾负离子(ESI-)和多反应监测模式(MRM)进行MS测定。结果表明:杀虫双添加水平为0.05、0.10和0.20mg/kg时,回收率为65.3%～91.5%;相对标准偏差为7.8%～11.8%;方法检出限为3.0μg/kg。本方法仅需约1min的检测时间,且具有很高的灵敏度和准确度,能够满足草莓及其制品中残留杀虫双的快速、高灵敏检测分析。
A ultra performance liquid chromatography with electrospray tandem mass spectrometry （UPLC-MS-MS） method was developed for the quantitative determination of bisultap residue in raw strawberry and its processed products. The samples were extracted by methanol-water （1：1, V/V） without any purifying treatments, were separated by ACQUITY UPLC HSS T3 UPLC column with equal gradient mobile phase, and detected by electrospray tandem mass spectrometry with ES- and multiple reaction monitoring （MRM） modes. Results showed that the mean recoveries range from 65.3% to 91.5% with relative standard deviations in the range of 7.8%-11.8% when the spiked concentrations of bisultap are 0.05, 0.10 and 0.20 mg/kg respectively, and the limit of detection is 3.0 la g/kg. The detection operation can be completed within 1 minute with high sensitivity and accuracy, and is suitable for the fast qualitative and quantitative detection for bisultap residue in strawberry samples.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第5期352-354,共3页
Food Science
