人乳头瘤病毒基因分型方法的建立和应用

Human papillomavirus genotyping:Establishment and application of DNA array method

目的建立一种简便快捷、灵敏度高、经济实用的人乳头瘤病毒(HPV)基因分型的低密度基因芯片技术,并对该方法进行评价。方法用低密度基因芯片技术对355例疑似HPV感染女患者检测HPV并分型;用杂交捕获Ⅱ代(HCⅡ)法进行评价,并用该法对珠江三角洲地区的730例标本进行HPV分型检测。结果355例疑似样本中,低密度基因芯片技术和HCⅡ法分别检出211例(59.4%)和222例(62.5%)阳性标本,符合率达94.1%(334/355)。低密度基因芯片技术共检测出15种常见高危型和5种低危型,其中16、52、58、56型检出率较高。结论低密度基因芯片技术能具体分型和检测混合感染,HPV检测与细胞学检测结合,对宫颈癌筛查有重要意义。

Objective To establish a convenient, fast, economic and hypersensitive low-density DNA array method to detect the genotypes of human papillomavirus （HPV） and evaluate its application in clinic services. Methods HPV in cervical swab samples from 355 suspected female patients collected in gynaecology and obstetrics clinic were genotyped by hybrid capture （HC） Ⅱ method and low-density DNA array simultaneously. HPV in 730 clinic samples from the area of Pearl River delta were genotyped by low-density DNA array. Results Among 355 suspected samples positive HPV-DNA were detectable in 211 （59.4%） samples by DNA array and 222 （62. 5% ） samples by HCⅡ method. The concordance rate between the two assays was 94.1%. In the HPV genotypes 15 high-risk type and 5 low-risk type were detected by low-density DNA array. The most common high-risk types were HPV-16, 52, 58 and 56. The peak age of HPV infection was 26-30 years. The distribution of genotypes was different from various degree of cervical changes. Conclusion Either single type or multiple type of HPV infection can be detected by low-density DNA array. The combination of HPV detection with cytology detection will provide instruction for cervical cancer screening.