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Reversal of Apoptotic Resistance by Lycium barbarum Glycopeptide 3 in Aged T Cells 被引量:12

Reversal of Apoptotic Resistance by Lycium barbarum Glycopeptide 3 in Aged T Cells
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摘要 Objective To study whether Lycium barbarurn glycopeplide 3 (LBGP3) affects T cell apoptosis in aged mice. Methods LBGP3 was purified with DEAE cellulose and Sephadex columns. Apoptotic "sub-G1 peak" was detected by flow cytometry and DNA ladder was resolved by agarose gel electrophoresis. Levels of IFN-7 and IL-10 were measured with specific kits and mRNA expression was detected by RT-PCR. Apoptosis-related proteins of FLIP, FasL, and Bcl-2 were determined by Western blotting. Results LBGP3 was purified from Fructus Lycii water extracts and identified as a 41 kD glycopeptide. Treatment with 200 p.g/mL LBGP3 increased the apoptotic rate of T cells from aged mice and showed a similar DNA ladder pattern to that in young T cells. The reversal of apoptotic resistance was involved in down-regulating the expression of Bcl-2 and FLIP, and up-regulating the expression of FasL. Conclusion Lycium barbarum glycopeptide 3 reverses apoptotic resistance of aged T cells by modulating the expression of apoptosis-related molecules. Objective To study whether Lycium barbarurn glycopeplide 3 (LBGP3) affects T cell apoptosis in aged mice. Methods LBGP3 was purified with DEAE cellulose and Sephadex columns. Apoptotic "sub-G1 peak" was detected by flow cytometry and DNA ladder was resolved by agarose gel electrophoresis. Levels of IFN-7 and IL-10 were measured with specific kits and mRNA expression was detected by RT-PCR. Apoptosis-related proteins of FLIP, FasL, and Bcl-2 were determined by Western blotting. Results LBGP3 was purified from Fructus Lycii water extracts and identified as a 41 kD glycopeptide. Treatment with 200 p.g/mL LBGP3 increased the apoptotic rate of T cells from aged mice and showed a similar DNA ladder pattern to that in young T cells. The reversal of apoptotic resistance was involved in down-regulating the expression of Bcl-2 and FLIP, and up-regulating the expression of FasL. Conclusion Lycium barbarum glycopeptide 3 reverses apoptotic resistance of aged T cells by modulating the expression of apoptosis-related molecules.
出处 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2008年第3期212-217,共6页 生物医学与环境科学(英文版)
基金 This research was supported by the National Basic Research Program of China (No. 2007CB507406) National Natural Science Foundation of China (No. 30600659)
关键词 Lycium barbarum glycopeptide 3 Aged T cells CYTOKINES APOPTOSIS SENESCENCE Lycium barbarum glycopeptide 3 Aged T cells Cytokines Apoptosis Senescence
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