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改进的稳定小分子量RNA(LMW)指纹图谱技术 被引量：1

Improvement of Stable Low Molecular Weight RNA (LMW) Fingerprint Atlas Technique
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摘要 通过对稳定小分子量RNA这项技术进行改进,使其与以往的分离技术相比,在时间上缩短到4.5 h,最高电压降低到1400V,胶板的长度降到30 cm;使其可操作性更强,更加适用于大量的样品的分析。 Low molecular weight RNA molecule fingerprint atlas technique was improved, as compared with previous separation technique; the time was reduced to 4.5 h, and the maximum voltage decreased to 1 400 V, and the length of gel was reduced to 30 cm, these made the operation more maneuverable, and more suitable to analyze a lot of samples.
作者 满朝新 王浩 陈文峰 陈文新
机构地区 中国农业大学生物学院农业部农业微生物资源及其应用重点开放实验室
出处 《微生物学杂志》 CAS CSCD 2008年第3期1-3,共3页 Journal of Microbiology
基金 自然科学基金面上项目(30670001)
关键词 稳定低分子量RNA 梯度电泳 根瘤菌 stable low molecular weight RNA gradient electrophoresis nodule bacteria
分类号 Q93-3 [生物学—微生物学]
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参考文献6

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同被引文献12

  • 1COLWELL R R.Polyphasic taxonomy of the genus Vibrio:numerical taxonomy of vihrio cholerae,Vibrio parahaemolyticus,and related Vibrio species[J].J Bacteriol,1970,104:410-433.
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  • 3H(O)FLE M G.Transfer RNAs as genotype fingerprints of eubacteria[J].Arch Microbiol,1990,153:299-304.
  • 4CRUZ-S(A)NCHEZ J M,VEL(A)ZQUFZ E,MATEOS P F,et al.Enhancement of resolution of low molecular weight RNA profiles by staircase-electrophoresis-electrophroesis[J].Electrophroesis,1997,18:1909-1911.
  • 5ENCARNA VEL(A)ZQUEZ,RA U1 RIVAS,MAR(I)A DEL VILLAR,et al.A new approach for separating low-molecular-weight RNA molecules by staircase electrophoresis in non-sequencing gels[J].Electrophoresis,2006,27:1732-1738.
  • 6ORME NO E,TORRFS R,MAYO J,et al.Phuaseolus lunatus is nodulated by a phosphate solubilizing strain of Sinorhizobium meliloti in a Peruvian soil[J].Developments in Plant and Soil Sciences,2007,102:143-147.
  • 7VEL(A)ZQUEZ E,CRUZ-S(A)NCHEZ J M,MATEOS P F,et al.Analysis of stable low-molecular-weight RNA profiles of members of the family Rhizobiaceae[J].Applied and Enviromental Microbiology,1998,64:1555-1559.
  • 8YOUNG J M,KUYKENDALL L D,MARTTNFZ-ROMERO E,et al.A revision of Rhizobium Frank 1889,with an emended description of the genus,and the inclusion of all species of Agrobacterium Conn 1942 and Allorhizobium undicola de Lajudie et al.,1998 as new combinations:Rhizobium radiobacter,R.rhizogenes,R.rubi,R.undicola and R.vitis[J].International Journal of Systematic and Evolutionary Microbiology,2001,51:89-103.
  • 9VEL(A)Z1QUEZ E,PALOMO J L,LASTRA B,et al.Rapid identification of Agrobacterium species by staircase electrophoresis of low molecular weight RNA profiles[J].Eur J Plant Pathol,2001,107:931-938.
  • 10VEL(A)ZQUEZ E,IGUAL J M,WILLLEMS A,et al.Mesorhizobium chacoense sp.Nov.,a novel species that nodulates Prosopis alba in the Chaco Arido region (Argentina)[J].Int J Syst Evol Micrbio1,2001,51:1011-1021.

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微生物学杂志
2008年 第3期

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