ApoB基因多态性与长沙汉族人群脑出血的关系及其对血脂水平的影响

Effect of apoB polymorphism on plasma lipid levels and cerebral hemorrhage in Changsha Han Chinese

目的:分析载脂蛋白B(apolipoprotein B,apo B)基因C7673T多态性与长沙地区汉族人群脑出血的关系及其对血脂水平的影响。方法:采用聚合酶链式反应-限制性片段长度多态性分析法(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)和DNA直接测序法检测130例脑出血患者和100例正常对照者的apoB基因C7673T多态;并用氧化酶法测定其血清甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白(high density lipoprotein-cholesterol,HDL)、低密度脂蛋白(low density lipoprotein-cholesterol,LDL)水平;酶联免疫吸附法测定脂蛋白(a)[lipoprotein(a),LP(a)]浓度;免疫比浊法测定apoB100及apoAI浓度。结果:(1)脑出血组和对照组apoB基因C7673T多态T等位基因频率分别为0.108和0.040,脑出血患者组apoB基因C7673T多态T等位基因频率显著高于对照组(P<0.01)。(2)脑出血组apoB基因C7673T多态T/C基因型较C/C基因型的TC和LDL-C显著增高,而HDL-C显著降低(P<0.05),其它指标差异无统计学意义(P>0.05)。结论:apoB基因C7673T多态可能与长沙地区汉族人群脑出血有关,且可能通过改变血脂水平影响脑出血的发生发展。

Objective To explore the effect of apoB polymorphism on plasma lipid levels and cerebral hemorrhage in（CH）Changsha Han Chinese.Methods One hundred thirty CH patients and 100 normal people were involved.C7673T polymorphism of apoprotein B was analyzed by PCR-restriction fragment length polymorphism（PCR-PFLP）;and the triglyceride（TG）,total cholesterol（TC）,high density lipoprotein-cholesterol（HDL）,and low density lipoprotein-cholesterol（LDL）levels were examined by oxidase method.The serum level of lipoprotein（a）was determined by immunology method.Results（1）Allele T frequencies of apoB C7673T in CH patients and the control group were 0.108 and 0.040,respectively.Allele T frequencies of apoB C7673T in the CH patients were significantly higher than those in the control group（P〈0.01）.（2）In the CH patients,the levels of TC and LDL-C of the T/C gene type were significantly higer than those of the C/C gene type,while the levels of HDL-C of the T/C gene type were significantly lower than those of the C/C gene type（P〈0.05）.Conclusion ApoB C7673T polymorphism may be related to cerebral hemorrhage,and the changing blood lipid level may increase the susceptibility of CH.