组蛋白甲基转移酶SMYD3在不同HBV表达水平肝癌细胞中的差异表达

SMYD3 expression differences in hepatoma cell lines with different HBV expression levels

目的:探讨HBV组蛋白甲基转移酶SMYD3在肝癌细胞中的表达与HBV感染状态相关性.方法:选取HBV阴性和HBV阳性的肝癌细胞株HepG2、HepG2.2.15,利用实时逆转录聚合酶链反应(real time RT-PCR)方法检测SMYD3 mRNA表达水平,Western blot检测细胞中SMYD3蛋白表达差异.结果:SMYD3 mRNA及蛋白在HepG2.2.15的表达水平显著高于HepG2(0.92±0.12 vs 0.18±0.05,0.28±0.03 vs 0.54±0.05,均P<0.01),有统计学差异.结论:HBV可能通过上调SMYD3途径促进肝癌的恶性生物学行为.

AIM： To evaluate correlation between histone methyltransferase SMYD3 expression in hepatoma and HBV infection. METHODS： SMYD3 mRNA expressions and SMYD3 protein expression levels in HBV-negative HepG2 and HBV-positive hepatoma cell line HepG2.2.15 were determined using real time PCR and Western blot, respectively. RESULTS： SMYD3 mRNA and protein levels were significantly higher in HepG2.2.15 than those in HepG2 （0.92 ± 0.12 vs 0.18 ± 0.05, 0.28 ± 0.03 vs 0.54 ± 0.05, both P 〈 0.01）. CONCLUSION： HBV may promote hepatoma cell malignancy through its SMYD3 up-regulating pathways.