摘要
目的:研究小檗碱(berberine,Ber)对人肝癌细胞株HepG2细胞中胰岛素诱导基因2(Insulin-induced gene 2,Insig-2)和维生素D受体(Vitamin D receptor,VDR)基因表达的影响,探讨其调血脂的作用机制。方法:采用RT-PCR技术检测不同浓度(1、3、10、30、100μmol/L)Ber处理24 h后及3μmol/L Ber处理不同时间(0、12、24、48、72 h)后HepG2细胞中In-sig-2和VDR mRNA的表达,并进行相关性分析。结果:不同浓度Ber处理HepG2细胞后,Insig-2及VDR基因的表达均明显上调,其上调效应随药物浓度的升高而逐渐增强,至3μmol/L浓度时达高峰,随后则逐渐减弱,两者的变化具有相关性,r=0.753(P<0.01);用3μmol/L浓度Ber处理HepG2细胞不同时间后,Insig-2及VDR基因的表达均明显上调,其效应随着时间的延长而不断增强,于48 h达峰值,随后减弱,两者的变化具有相关性,r=0.922(P<0.01)。结论:Ber调血脂的作用机制可能是通过上调VDR的表达,进而上调Insig-2基因表达,从而抑制肝脏中胆固醇的合成代谢。
AIM ： To observe the mRNA expressions of Insulin-induced gene 2 （Insig-2） and Vitamin D receptor （VDR） in HepG2 cell line and investigate the possible mechanism of berberine on regulating blood lipid. METHODS： HepG2 ceils were incubated with Bet at different concentrations （1, 3, 10, 30, 100 p, mol/L）, and incubated with 3 p, mol/L berberine for various times （0, 12, 24, 48, 72 h）. Total RNA was extracted and the mRNA expressions of Insig-2 and VDR from HepG2 ceils treated by berberine were quantified by RT-PCR. RESULTS： After exposure to gradient concentrations of berberine for 24 h, the mRNA expressions of Insig-2 and VDR in HepG2 ceils increased obviously peaked at 3 p, mol/L concentration, then the up-regulating effect declined gradually. The relative coefficient between them was 0.753 （P 〈 0.01 ）. After being treated with 3 p, mol/L berberine for different time, the mRNA expression of Insig-2 and VDR in HepG2 ceils significantly increased with the prolongation of the exposure time, peaked at 48 h, then declined at 72 h. The relative coefficient between them was 0.922 （p 〈 0.01 ）. CONCLUSION： Berberine can increase the mRNA expression of Insig-2 and VDR in HepG2 cell. The possible mechanisms of its effects for lowering blood lipid may be relevant to up-regulating VDR and Insig-2 gene expression and inhibiting cholesterol synthesis.
出处
《中成药》
CAS
CSCD
北大核心
2008年第7期965-968,共4页
Chinese Traditional Patent Medicine
基金
国家自然科学基金资助项目(30600812)
