摘要
hKv4.3基因是形成瞬时外向钾电流Ito的主要分子基础,它在心脏和神经细胞中大量表达,但在其它组织中则未见大量表达.为了研究hKv4.3表达在基因水平的调节,将hKv4.3基因的5′非翻译区的一段序列(+2^+160,称之为S160)克隆到报告质粒中,进行瞬时表达.发现S160对hKv4.3基因的启动子和SV40的启动子都有强烈的抑制作用,没有方向特异性,但却有位置特异性.经删除突变分析,在S160片段中发现了一个抑制元件S(GAGGGGTTAA),它位于hKv4.3基因中转录起始位点下游20~30bp处.在此基础上,用RT-PCR方法对mRNA进行定量分析,初步确认这个抑制元件对蛋白表达的抑制过程是在翻译水平上.
hKv4. 3 is the main molecular basis of transient outward K^+ current (lto). Its expression is found in abundance in heart and brain, but with no detectable expression in other tissue. In order to define the gene regulation of hKv4. 3 expression, we cloned the sequence( +2— +160, S160) in the 5'UTR of hKv4. 3 gene into the report plasmid, and it was transiently expressed. It was found that S160 repressed the activity of promoter of hKv4. 3 and SV40, moreover, its repression function with position-specificity but without orientation-specificity. Through the analysis of deletion mutant, we found an repressor element S (GAGGGGrITAA) locating in the downstream region( +20— +30 bp) of TSS. We analyzed mRNA quantity with RT-PCR method, and think that the repressor element S maybe represses the expression in translation level.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2008年第7期1384-1389,共6页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:30170215)资助