P504S、p63、34βE12在前列腺不同病变诊断中的意义

Expression and significance of p504S,p63,34βE12 in prostate lesions

目的探讨P504S、p63、34βE12在良性前列腺增生、不典型腺瘤样增生、前列腺上皮内瘤变和前列腺腺癌诊断中的意义。方法收集102例前列腺不同病变的常规石蜡标本,光镜下按WHO标准分类,应用免疫组织化学二步法,观察P504s、p63、34βE12的表达。结果良性前列腺增生、不典型腺瘤样增生的腺泡和导管周围34βE 12和p63均为(+),而P504S均(-)。低级别PIN及高级别PIN的腺泡和导管周围34βE 12和p63均为(+),而增生的腺上皮P504S分别为7.7%(2/26)、33.3%(1/3)呈局灶性阳性。前列腺腺癌18例中,34βE12和p63均为(-),P504S有2例呈局灶性阳性(11.1%),15例P504S呈阳性(83.3%)。结论P504S是前列腺腺癌敏感而特异性的标记物,联合检测P504S、p63、34βE12可提高前列腺腺癌的诊断准确率,对前列腺穿刺标本进行不同病变的鉴别诊断更有帮助。

Objective To explore the diagnostic significance of P504S, p63 and 3413E12 in the benign prostatic hyperplasia（BPH）, atypical adenomatous hyperplasia（AAH）, prostatic intraepithelial neoplasia（PIN） and prostatic adenocarcinoma. Methods We collected 102 specimens with different prostate lesions, and reclassifed them according to the WHO. Immunohistochemical staining for P504S, p63 and 34βE12 was performed by using En Vision. Results Continous positive expression of 34βE12 and p63 was found in the aeini and ducts of the BPH and AAH, but P504S staining presented negative reaction. Continous positive expression of 34βE12 and p63 was found in the aeini and ducts of the low PIN and high PIN, and a part of hyperplastie glandular epithelia presented locally expression for P504S; 18 prostatic adenoeareinoma cases was negative for 34βE12 and p63, 2 cases presented focally positive reaction by P504S staining, and P504S staining was positive in 15 out of 18. Conclusions P504S is a sensitive and specific marker prostate adenoeareinoma. Combined detection of P504S, p63 and 34βE12 can be most helpful for diagnosis of prostate adenoeareinoma. It is of greater benefit in aiding the differential diagnoses of prostate lesions in needle biopsy speeimem.