细胞色素P4501A1基因多态与肾癌遗传易感性的关系

Relationship between CYP1A1 gene single nucleotide polymorphism and genetic susceptibility of renal cancer

目的：探讨细胞色素P4501A1（CYP1A1）酶的基因多态与肾癌易感性的关系。方法：采用病例对照设计，以175名肾癌患者为病例组，200名来患任何肿瘤的健康人为对照组。应用聚合酶链反应限制性片段长度多态性（PCR—RFLP）技术，检测CYP1A1基因A4889G（M2）、C4887A（M4）的多态性。结果：病例组M2、M4备基因型分布与对照组相比无统计学差异。携带M2／M2基因型者与肾癌的发生呈正相关（OR=2．225，95%CI：1．134-4．365，P=0．020）。结论：CYP1A1基因M2／M2基因型可能是肾癌的遗传危险因素。

Objective：To evaluate the relationship between the polymorphism of CYP1A1 gene and the genetic susceptibility to renal cancer. Methods： A case-control study was conducted in 175 renal cancer patients and 200 healthy control subjects to investigate the role of CYP1A1 gene A4889G（M2） and C4887A（M4） polymorphisms in renal cancer. PCR-RFLP was used to identify the genotypes of polymorphism. Results： The frequencies of A4889G and C4887A genotypes were not significantly different between RCC cases and healthy controls. M2/M2 genotype was positively correlated with the incidence of renal cancer （0R=2.225 [95%CI=l.134-4. 365,P=0. 020]）. Conclusion： M2/M2 genotypeofCYP1A1 gene may be a risk factor for renal cancer.