决明子的不同提取物及炮制品对四氯化碳致小鼠急性肝损伤的保护作用

Protective Effects of Different Extracts and Processed Products of Semen Cassiae against the Acute Liver Injury Induced by CCl_4 in Mice

目的观察不同极性溶剂的系列提取物、总蒽醌中的游离型和结合型蒽醌(蒽醌苷)及不同的炮制品的保肝作用。方法将KM小鼠随机分组,分成正常组、模型组、联苯双酯组(0.1 g/kg)、各决明子组(11 g生药/kg),连续给药9 d,末次给药后约10 h,除正常组外,其它各组均腹腔注射0.15%四氯化碳溶液10 m l/kg。16 h后采用比色法测定血清中转氨酶ALT,AST的含量。结果以不同极性溶剂提取的5种决明子提取物中,除石油醚部分外,其余各组均有显著降低转氨酶活性的作用;游离型和结合型蒽醌均有显著的降低转氨酶活性作用;决明子的生、炒两类炮制品也同样有显著的降酶作用,但生品略优。结论总蒽醌类成分是保肝降酶的主要活性成分;除总蒽醌外尚有其他多种保肝降酶的活性成分,有待于对不同提取物作进一步的活性成分追踪研究;决明子的生、炒两类炮制品在用于保肝降酶活性时以生品为佳。

Objective To investigate the protective effects of different extracts and processed products of Semen Cassiae on the acute liver injury induced by CCl4, Methods KM mice were divided into normal group, control group, biphenyl dimethylester group （0.1 g/kg） and Semen Cassiae group （11g crude drug/kg） , which were processed by intragastric administration for 9 days. 0.15% CCl4 oil solution （ 10 ml/kg, diluted with plant oil） was injected into the mice of all groups but normal group by intraperitoneal injection 10hr after last administration. The contents of ALT and AST in the serum were determined by chromatometry 16hr after administration of CCl4. Results The five kinds of extracts extracted by different heteropolarity dissolvents but ligarine extract, the free and conjugated anthraquinones significantly decreased the activities of aminopherase. The processed products of Semen Cassiae, the crude and the parched, significantly decreased the activities of aminopherase too. The effect of the crude on the decrease of aminopherase activities was prior to the parched slightly. Conclusion All of the anthraquinones in Semen Cassiae was the primary active component at the protective effects on liver and decrease of the aminopherase activities and there would be other active components besides all anthraquinones. The follow - up of active components in other extracts but ligarine extract would be urgent at protective effects on liver in the future. The crude may be optimal between two processed products of semen cassiae if Semen Cassiae is used for the protective aim on liver and decrease of aminopherase activities.