肺癌患者PTEN基因启动子高甲基化的检测

Detection of PTEN gene promoter hypermethylation in lung cancer patients

目的探讨肺癌患者组织、外周血浆及支气管肺泡灌洗液(BALF)中张力蛋白同源的磷酸酶基因(PTEN)启动子异常基因化状况及其在肺癌诊断中的价值。方法用甲基化特异性PCR方法检测组织、血浆及BALF中的PTEN基因启动子区CpG岛甲基化。结果45例肺癌患者中,PTEN基因启动子异常甲基化率组织为26.67%(12/45)、血浆为15.56%(7/45),BALF为22.22%(10/45);而非肺癌组织、正常对照血浆、非肺癌患者BALF中未检出甲基化;血浆、BALF中甲基化改变与肿瘤组织甲基化状况显著相关(P<0.01)。结论血浆、BALF中PTEN基因异常甲基化改变的检测在肺癌的早期特异诊断等方面有一定的价值。

Objective To investigate the status and diagnostic value of aberrant phosphatase and tensin homology deleted on chromosometen（PTEN） gene promoter in tissues, peripheral plasma and BALF of lung cancer patients. Methods We analyzed the methylation of PTEN gene in tissues, peripheral plasma and BALF by methylation specific-PCR. Results The frequency of methylation of promoter of PTEN gene was 26. 67% （ 12/45 ） in lung cancer tissues, 15.56% （7/45） in peripheral plasma, 22. 22% （10/45） in BALF, no methylation product was found in lung tissues without cancer, normal plasma and BALF controls （P 〈 0. 01 ）. There were significantly coincident in patients tumor tissues, plasma and BALF. Conclusion Detection of aberrant methylation of PTEN promoter in plas- ma and/or BALF may contribute to a definite value in early prognostic in lung cancer.