锌对小鼠睾丸组织NO的含量及抗氧化作用的影响

EFFECT OF ZIN ON THE CONTENT OF NITRIC OXIDE IN THE TESTIS OF MICE AND ITS ANTIOXIDATION

[目的]通过研究锌对雄性小鼠睾丸的抗氧化作用,探讨高锌引起的睾丸生精小管受损的机制。[方法]将雄性小鼠随机分为4组,对照组用普通饲料喂养,实验组分为3组:分别用含不同剂量的高锌饲料喂养,检测4组小鼠的睾丸的丙二醛(MDA)含量、超氧化物歧化酶(SOD)、一氧化氮(NO)、一氧化氮合酶(NOS)、睾丸系数等指标并对睾丸进行光镜结构观察。[结果]高锌中、高剂量组小鼠睾丸组织MDA、NO含量升高,与对照组相比较差异有统计学意义(P﹤0.05);高锌中、高剂量组小鼠睾丸组织SOD活性下降,与对照组相比差异有统计学意义(P﹤0.05,P﹤0.01);高锌各剂量组小鼠睾丸组织NOS活性升高,与对照组相比差异有统计学意义(P﹤0.05);4组睾丸系数差异无统计学意义;高锌各剂量组小鼠睾丸生精小管受到了到了不同程度的损害,饲料中含锌量越高,损害程度越重。[结论]高锌对雄性小鼠的睾丸有损害,高锌引起的睾丸组织的脂质过氧化作用增强、SOD活力减弱、,以及NOS活性增加引起NO过量产生可能是其重要原因。

[ Objective] To study on the antioxidation of zin on the testis of mice, and to explore the impaired mechanism of seminiferous tubule in testis induced by zinc. [Methods] The mice were divided into 4 groups. The control group was feed with feedstock, while the experimental groups were divided into 3 groups which were feed with different dosages of high zinc feedstock. The level of maleic Dialdehyde （MDA） and nitric oxide （NO）, the activity of superoxide dismutase （SOD） and nitric oxide synthase （NOS） and the coefficient of testicles in the four experimental groups were detected. [ Results] When compared with control group, the level of MDA and NO was significantly higher （P 〈 0.05）, but the activity of SOD was significantly lower （P 〈 0.05, P 〈 0.01 ） in groups with middle and high dosages of zinc feedstock. The activity of NOS was significantly increased in all experimental groups compared with the controls （P 〈 0.05）. No significant difference was found among coefficients of testicles of the four experimental groups; The seminiferous tubule in testis in experimental groups were damages in different degree, and the degree of damage was arised with the increasing content of zinc in feedstock. [Conclusion] High zinc can damage to the testis, and increase the lipid peroxidation of testicular issues, decrease the activity of SOD, and increase the activity of NOS which induces the over producing of NO.