精子提取液对绵羊同异种核移植重构胚的激活效果

Effects of Ovine Sperm Extract on the Development Competence of Homogeneous and Heterogeneous Embryos Reconstructed by Nuclear Transfer

应用绵羊精子提取物体外激活绵羊体细胞核移植胚和山羊-绵羊异种核移植胚,比较了不同浓度的绵羊精子提取物对绵羊同种和异种重构胚的激活作用,并与传统的化学激活方法对重构胚的卵裂率、囊胚率和囊胚细胞数做了比较。结果表明,绵羊精子提取物可以有效地激活绵羊体细胞核移植胚和山羊-绵羊异种核移植胚,每一重构胚注射3 pL浓度为5 mg/mL的绵羊精子提取物,对同种和异种胚的发育效果均为最好,并显著高于其他试验组(P<0.05)。注射绵羊精子提取物对绵羊-山羊异种核移植胚的卵裂率、囊胚率和囊胚细胞数的影响,与离子霉素联合6-DAMP激活法差异不显著(P>0.05)。注射绵羊精子提取物对绵羊同种核移植胚的卵裂率和囊胚率的影响,与离子霉素联合6-DAMP激活法差异也不显著(P>0.05),但精子提取物注射法的绵羊核移植胚的囊胚细胞数显著(P<0.05)高于化学激活法的细胞数(72.4 vs 65.2)。

Ovine sperm extract （OSE） was used to activate ovine somatic cell nuclear transfer （SCNT）embryos and goat-ovine interspecies somatic cell nuclear transfer（ISCNT） embryos. The effects of different dose of OSE activation on the development capacity of the reconstructed embryos were compared according to the cleavage rate, blastocyst rate and blastocyst cell number. The results showed that OSE could effectively activate the SCNT and the ISCNT embryos and the optium concentration of OSE was 5 mg/mL, the activation effect was significantly（P〈0. 05） better than other groups. The effects of OSE activation or chemical activation （using ionmiein and 6- DMAP） on the development capacity of the reconstructed embryos were evaluated, the results indicated that there was no significant difference in blastocyst rate and blastoeyst cell number when the ISCNT embryos were activated by using the OSE or the chemical activation method and there was no significant difference in blastocyst rate when the SCNT embryos were activated, however, there was a significant increase（P〈0.05） in blastocyst cell number when the SCNT embryos was activated by using OSE compared with the chemical activation method（72.4 vs 65.2 ,P〈0.05）.