金鱼草嫩茎组织培养及无性系建立的研究

Tissue culture and regeneration clone for young stem of Antirrhinum majus

以金鱼草变异植株的嫩茎为外植体,采用不同培养基对其愈伤组织的诱导与分化、不定芽生根、试管苗移栽和扦插等进行研究,以建立金鱼草变异植株的无性系。试验结果表明:MS+6-BA 0.8mg/L+IBA 0.1mg/L+2,4-D 0.5mg/L+蔗糖30g/L是诱导金鱼草嫩茎愈伤组织的理想培养基,MS+AgNO30.6mg/L+6-BA 0.2mg/L+NAA 0.1mg/L+蔗糖30 g/L是诱导愈伤组织和不定芽分化的理想培养基,1/3MS+IAA 0.6mg/L+蔗糖10g/L是金鱼草试管苗生根培养和生根继代培养的理想培养基;炉灰渣是试管苗移栽扦插的最适合基质,移栽成活率为96%,扦插成活率为93%。移植于花坛的试管苗具有保持有利变异的特点。

The tender stem of mutation Antirrhinum majus was used as explants, the induction and differentiation of callus, rooting of adventitious bud in different culture medium, and the transplantation and graftage of plantlet were studied to establish the regeneration clone for mutation of Antirrhinum majus. The results showed that the optimum culture medium for callus induction was MS ＋6-BA 0.8mg/L＋IBA 0. 1mg/L＋ 2,4-D 0. 5mg/L, culture medium MS＋AgNO30. 6mg/L＋6-BA 0.2mg/L＋NAA 0. 1mg/L＋sugar 30 g/L was suitable for differentiation of callus and adventitious bud; culture medium 1/3MS＋IAA 0.6mg/L＋sugar 10g/L was ideal for rooting of seedling and subculture. When cinder was used as the best matrix for transplantation and graftage of plantlet, the survival rate of transplantation could reach 96% and that of graftage was 93%. The trasplanting plantlet to flower bed could keep favorable variation.