胸腺肽α_1对神经外科肿瘤患者T淋巴细胞亚群和NK细胞的影响

Effect of thymic peptide α_1 on perioperative cellular immune function in patients with brain neoplasms

目的了解胸腺肽α1对神经外科肿瘤患者围术期T淋巴细胞亚群和自然杀伤(NK)细胞数量的影响。方法选择30例神经外科肿瘤手术的患者,随机均分为研究组和对照组,研究组麻醉前用胸腺肽α1,对照组未使用。分别于麻醉前、手术1 h、术毕和术后5 d抽取外周静脉血,用流式细胞仪检测T淋巴细胞亚群(CD3+、CD4+、CD8+)和NK细胞(CD56+)数量的变化。结果研究组和对照组手术1 h CD3+、CD4+、CD4+/CD8+、NK细胞数量均较麻醉前明显下降(P<0.05或P<0.01),研究组下降幅度较对照组小(P<0.05),术毕研究组和对照组各指标有所回升,但研究组回升的幅度较对照组大,术后5 d研究组和对照组各指标均回到麻醉前水平。结论胸腺肽α1减小了神经外科肿瘤患者围术期T淋巴细胞亚群和NK细胞下降的程度,能明显减轻细胞免疫功能的抑制。

Objective To study the effects of thymic peptide α1 on perioperative cellular immune function in patients with brain neoplasms. Methods Thirty patients of brain neoplasms were randomly divided into experimental group and control group. Thymic peptide α1 was used in experimental group before anesthesia. Peripheral venous blood samples were taken before anesthesia, at 1 h after incision,at end of operation and on the 5th day after operation. CD3^＋ ,CD4^＋ ,CD8^＋ and NK cells were measured by flow cytometry. Results One hour after incision,CD3^- ,CD4^＋ ,CD4^＋/ CD8^＋ and NK cells in both groups were significantly decreased （P〈0.05）. The extent of decrease in experimental group was smaller than that in control group （P〈0. 05）. The values recovered at the end of operation in two groups. The extent of recovery in experimental group was larger than that in control group. The values on the 5th day after operation recovered to the level of before anesthesia in both groups. Conclusion Thymic peptide α1 can reduce the decrease of T-lymphocytes subsets and NK cells,and thus improve the cellular immune function in surgical patients with brain neoplasms.