几种蚜虫对MDMV传毒效率及其口针中病毒附着位点(VAS)的免疫荧光标记

TRANSMISSION RATE AND LOCATION OF MAIZE DWARF MOSAIC VIRUS ATTACHMENT SITES (VAS) IN THE STYLETS OF APHIDS BY IMMUNOFLUORESENCE LABEL

本研究在测定了５种蚜虫对玉米矮花叶病毒（ＭＤＭＶ）传播效率的基础上，采用免疫荧光（ＦＩＴＣ）标记方法，观察到蚜虫口针中ＭＤＭＶ附着位点（ＶＡＳ）的存在。结果发现不同蚜虫之间的传毒效率及ＶＡＳ有一定的差异。其中，以麦二叉蚜（Ｓｃｈｉｚａｐｈｉｓｇｒａｍｉｎｕｍ）的传毒效率最高，达６６．８％。并且其ＶＡＳ也最明显，位于口针末端约５０μｍ处。通过试验发现，尽管在ＶＡＳ存在的情况下，用提纯的不含ＨＣ的ＭＤＭＶ进行蚜虫传毒试验，其传毒率为零。只有当ＨＣ与ＭＤＭＶ共同存在的情况下才可有效传播。至于蚜虫是如何释放病毒的还不清楚。根据试验结果初步认为，蚜虫对ＭＤＭＶ的传播是一个“识别—吸附—释放”的过程。

Transmission rate and location of maize dwarf mosaic virus attachment sites (VAS) in the stylets of aphids by immunofluoresence label were observed. The results showed that the rate of transmission of MDMV is different between five aphids, including schizaphis graminum, Rhopalosiphum padi, Rhopalosiphum maidis, Macrosiphum avenae, and Myzus persicae Among them Schizaphis graminum has the highest transmission rate. MDMV VAS is different from different aphids stylet. VAS is quite obvious in S.graminum stylet, locating on the maxillae at about 50 μm from the tip. In addition, experiment shows that the HC Pro transmiasion of MDMV is in accordance to the procedure of"recognition adhesion release".