牛膝多糖对小鼠肝癌H22细胞bcl-2和Fas基因表达的影响

EFFECTS OF ACHYRANTHES BIDENTATA POLYSACCHARIDES ON THE EXPRESSION OF BCL-2 AND FAS IN HEPATOCARCINOMA H22 CELLS

目的:研究牛膝多糖(ABPS)对肝癌H22细胞bcl-2和Fas基因mRNA表达的影响。方法:用肝癌H22细胞进行体外培养,采用MTT法检测不同浓度ABPS对H22细胞的生长抑制作用;RT-PCR检测bcl-2和Fas基因mRNA表达的变化。结果:ABPS对肝癌H22细胞具有显著生长抑制作用,并呈量效关系;bcl-2基因mRNA表达水平随着ABPS浓度的增加而下降,Fas基因mRNA表达水平随着ABPS浓度的增加而上升。结论:ABPS可抑制H22细胞增殖并诱导其凋亡,其作用机制可能与下调bcl-2基因mRNA表达、上调Fas基因mRNA表达有关。

Objective： To investigate the Effects of Achyranthes bidentata polysaccharides on Hepatocarcinoma cell line H22 and the expression of bcl-2 and Fas.Methods：Hepatocarcinoma cell line H22 were used in vitro cell culture. The inhibitory effects of ABPS on H22 cell line were assayed with MTT test. The expression of bcl-2 and Fas were detected by RT-PCR. Results： ABPS can obviously inhibit the H22 cell growth with dose dependent. The expression of bcl-2 mRNA was decreased and the expression of Fas mRNA was increased with increasing of ABPS concentration. Conclusion：ABPS may inhibit H22 cells proliferation and induce apoptosis. The possible mechanism may be through down-regulation the expression of bcl-2 and up-regulation the expression of Fas.