超高效液相色谱-串联质谱法对鳗鱼及制品中禾草丹残留量的测定

Determination of the thiobencarb in eel and eel products by UPLC/Electrospray Ionization Tandem Mass Spectrometry

本文建立了用超高效液相色谱-串联质谱法对鳗鱼及制品中禾草丹的检测方法。采用乙腈对样品中的禾草丹进行提取,浓缩后用固相萃取SPE小柱、氧化铝、无水硫酸钠进行净化,最后用超高效液相色谱-质谱联用法进行分离和定性定量分析。方法线性相关系数为0.9966,5-20μg/kg,加标样品的回收率在75%-85%之间,相对标准偏差在8.3%-12.7%,定量检出限可达到0.5μg/kg,该方法具有快速、高效、灵敏度高,符合出口检测限量的要求。

In the paper the analytical method for the determination of thiobencarb pesticide residues in eel and eel products by UPLC-Electrospray Ionization Tandem Mass Spectrometry were established. Thiobencarb residues were extracted with acetonitrile,followed by clean up with SPE column added with Al2O3 and Na2SO4,and qualitative and quantitative were analysed by UPLC-MS. The linear coefficient of this method was 0.9966,for spiked samples （range from 5 to 20μg/kg）,the recoveries were 75%-85%, the RSDs were 8.3%-12.7%,the detection limits were 0.5 μg/kg,The method was effective, fast and high sensitivity ,meet the request of detection of export.