基因枪转移Bar基因获得抗除草剂(Basta)的糜子蔗克隆再生植株

Regenerated Plants Resistanted to Basta were Obtained ThroughIntroduction of BarGenes into〔(Sorghum×Sugarcane)×Millet Sorghum〕Using Particle Bombardment

用糜子蔗（ｓｏｒｇｈｕｍ×ｓｕｇａｒｃａｎｅ）×ｍｉｌｅｔｓｏｒｇｈｕｍ茎尖愈伤组织为受体，通过基因枪轰击将Ｂａｒ基因转入糜子蔗细胞；用除草剂Ｂａｓｔａ含量分别为１、２５、５、１０ｍｇ／Ｌ的８１１４诱导与分化培养基上选择抗性愈伤组织；在Ｂａｓｔａ含量为１与１５ｍｇ／Ｌ筛选培养基上，选择出３个快速增殖并大量再生绿苗的体细胞无性系（Ｃｌｏｎｅ），如ＢａｒＣ１、ＢａｒＣ２、ＢａｒＣ３；ＢａｒＣ１、Ｃ２再生植株用Ｂａｓｔａ含量为１、２５～５ｍｇ／Ｌ的溶液进行抗性检测，证明这些小植株具有极强的抗性。

The Bargenes were introduced into 〔(sorghum×sugarcane)×millet sorghum〕 callus cells using particle bombardment. The callus were cultured on the 8114 media with Basta, the Basta's concentration is 1, 25, and 5, 10mg/L.3 Clones with regenerated capacity were screened out on the media with Basta 1, 25mg/L, for example BarC1, BarC2, BarC3.The regenerated plant from BarC1、BarC2were cultured on the solution with Basta 15, 25, and 5mg/L, high resistance were obtained in these regenerated plants.