大鼠颌下腺促性腺激素释放激素及其mRNA的免疫组织化学与原位杂交研究

IMMUNOHISTOCHEMICAL AND IN SITU HYBRIDIZATION STUDY OF GnRH AND GnRH mRNA IN SUBMAXILLARY GLAND OF RATS

用免疫组织化学及原位杂交法，研究了促性腺激素释放激素及其ｍＲＮＡ在大鼠颌下腺的分布。结果显示，大鼠颌下腺的浆液性腺泡的上皮细胞，各级导管的上皮细胞及副交感神经节细胞均呈促性腺激素释放激素免疫反应阳性，阳性反应物质分布在胞质，胞核呈阴性反应。颌下腺的浆液性腺泡上皮细胞，各级导管上皮细胞同样被检测到很强的促性腺激素释放激素ｍＲＮＡ杂交信号。以上结果提示，大鼠颌下腺能自身合成促性腺激素释放激素，促性腺激素释放激素对消化功能可能有重要调节作用。

Distribution of GnRH and GnRH mRNA in submaxillary gland of male rats was studied by using immunohistochemical and in situ hybrydization methods. The results showed that the glandular epithelial cells of serous acinus,the epithelial cells of all gland ducts and parasympathetic ganglion cells appeared GnRH positive in immunoreaction.The positive substance was distributed in the cytoplasma with negative nuclei.The GnRH mRNA hybridized signal also can be detected in the epithelial cells of serous acinus and in the epithelial cells of all gland ducts. These results suggested that submaxillary gland of rats can synthesize GnRH, which may play an important role in the regulation of digestive function.