氧麻滴鼻液的导数分光光度法测定

The assay of ofloxacin and ephedrine nasal drops by derivative spectrophotometry

目的：建立氧麻滴鼻液的含量测定方法。方法：采用一阶和二阶导数分光光度法测定氧氟沙星和盐酸麻黄碱的含量，取２７８ｎｍ和２１６．２ｎｍ波长处的振幅值作为定量信息。结果：相关系数均为ｒ＝０．９９９９，平均回收率为９９．６％和９９．７％，ＲＳＤ为０．４％和０．６％。结论：该方法简便。

irst and second order derivative spectrophotometries were developed for the determination of ofloxacin and ephedrine hydrochloride. The absolute values of amplitude at 278 nm and 216.2 nm were used as the quantitative information respectively, the correlation coefficients were both 0.999 9 , the average recoveries were 100.1% and 99.7% , RSD were 0.6% and 0.5% respectively.